Knowledge Management System Of Institute of process engineering,CAS
| Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus | |
Sharshar, Moustafa Mohamed1,2; Samak, Nadia Abdrabou1,2,3; Ambreen, Sadaf4; Hao, Xuemi1,2; Mu, Tingzhen1; Maarouf, Mohamed5,6; Zheng, Chen1,2; Gao, Yibo1,2; Liu, Zhixia1; Jia, Yunpu1,2; Li, Xiangyuan1; Zhong, Wei7; Peh, Sumit1,2; Yang, Maohua1; Xing, Jianmin1,2
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| 2020-12-01 | |
| Source Publication | BIORESOURCE TECHNOLOGY
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| ISSN | 0960-8524 |
| Volume | 317Pages:11 |
| Abstract | Complicated production procedures and superior characteristics of nano-sized sulfur elevate its price to 25-40 fold higher than micrograde kind. Also, natural gas hydrogen sulfide levels are restricted because of its toxic environmental consequences. Thioalkalivibrio versutus is a polyextremophilic industrial autotroph with high natural gas desulfurization capability. Here, nanometric ( > 50 nm) sulfur bioproduction using T. versutus while desulfurizing natural gas was validated. Also, this production was enhanced by 166.7% via lowering sulfate production by 55.1%. A specially-developed CRISPR system, with 42% editing efficiency, simplified the genome editing workflow scheme for this challenging bacterium. In parallel, sulfur metabolism was uncovered using proteins mining and transcriptome studies for defining sulfate-producing key genes (heterodisulfide reductase-like complex, sulfur dioxygenase, sulfite dehydrogenase and sulfite oxidase). This study provided cost-effective nanometric sulfur production and improved this production using a novel CRISPR strategy, which could be suitable for industrial polyextremophiles, after uncovering sulfur pathways in T. versutus. |
| Keyword | Nanometric sulfur bioproduction Desulfurization Cell death-induced CRISPR Sulfur pathways Thioalkalivibrio versutus |
| DOI | 10.1016/j.biortech.2020.124018 |
| Language | 英语 |
| WOS Keyword | FED-BATCH ; GENOME ; PERFORMANCE ; CRISPR ; OXIDATION ; SEQUENCE ; PLATFORM ; PCR |
| Funding Project | National Science Foundation of China[21878307] ; National Science Foundation of China[31872633] ; National Science Foundation of China[31800030] ; National Key R&D Program of China[2020YFA0906800] ; CAS-TWAS (The World Academy of Sciences) |
| WOS Research Area | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS Subject | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| Funding Organization | National Science Foundation of China ; National Key R&D Program of China ; CAS-TWAS (The World Academy of Sciences) |
| WOS ID | WOS:000577077400008 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/42410 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Xing, Jianmin |
| Affiliation | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Coll Chem Engn, 19 A Yuquan Rd, Beijing 100049, Peoples R China 3.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt 4.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China 5.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 6.Suez Canal Univ, Fac Vet Med, Dept Virol, Ismailia 41522, Egypt 7.Chinese Acad Sci, Guangdong Prov Key Lab Synthet Genom, Shenzhen Key Lab Synthet Genom,Shenzhen Inst Adv, CAS Key Lab Quantitat Engn Biol,Shenzhen Inst Syn, Shenzhen 518055, Peoples R China |
| Recommended Citation GB/T 7714 | Sharshar, Moustafa Mohamed,Samak, Nadia Abdrabou,Ambreen, Sadaf,et al. Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus[J]. BIORESOURCE TECHNOLOGY,2020,317:11. |
| APA | Sharshar, Moustafa Mohamed.,Samak, Nadia Abdrabou.,Ambreen, Sadaf.,Hao, Xuemi.,Mu, Tingzhen.,...&Xing, Jianmin.(2020).Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus.BIORESOURCE TECHNOLOGY,317,11. |
| MLA | Sharshar, Moustafa Mohamed,et al."Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus".BIORESOURCE TECHNOLOGY 317(2020):11. |
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