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Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus
Sharshar, Moustafa Mohamed1,2; Samak, Nadia Abdrabou1,2,3; Ambreen, Sadaf4; Hao, Xuemi1,2; Mu, Tingzhen1; Maarouf, Mohamed5,6; Zheng, Chen1,2; Gao, Yibo1,2; Liu, Zhixia1; Jia, Yunpu1,2; Li, Xiangyuan1; Zhong, Wei7; Peh, Sumit1,2; Yang, Maohua1; Xing, Jianmin1,2
2020-12-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume317Pages:11
AbstractComplicated production procedures and superior characteristics of nano-sized sulfur elevate its price to 25-40 fold higher than micrograde kind. Also, natural gas hydrogen sulfide levels are restricted because of its toxic environmental consequences. Thioalkalivibrio versutus is a polyextremophilic industrial autotroph with high natural gas desulfurization capability. Here, nanometric ( > 50 nm) sulfur bioproduction using T. versutus while desulfurizing natural gas was validated. Also, this production was enhanced by 166.7% via lowering sulfate production by 55.1%. A specially-developed CRISPR system, with 42% editing efficiency, simplified the genome editing workflow scheme for this challenging bacterium. In parallel, sulfur metabolism was uncovered using proteins mining and transcriptome studies for defining sulfate-producing key genes (heterodisulfide reductase-like complex, sulfur dioxygenase, sulfite dehydrogenase and sulfite oxidase). This study provided cost-effective nanometric sulfur production and improved this production using a novel CRISPR strategy, which could be suitable for industrial polyextremophiles, after uncovering sulfur pathways in T. versutus.
KeywordNanometric sulfur bioproduction Desulfurization Cell death-induced CRISPR Sulfur pathways Thioalkalivibrio versutus
DOI10.1016/j.biortech.2020.124018
Language英语
WOS KeywordFED-BATCH ; GENOME ; PERFORMANCE ; CRISPR ; OXIDATION ; SEQUENCE ; PLATFORM ; PCR
Funding ProjectNational Science Foundation of China[21878307] ; National Science Foundation of China[31872633] ; National Science Foundation of China[31800030] ; National Key R&D Program of China[2020YFA0906800] ; CAS-TWAS (The World Academy of Sciences)
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
Funding OrganizationNational Science Foundation of China ; National Key R&D Program of China ; CAS-TWAS (The World Academy of Sciences)
WOS IDWOS:000577077400008
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42410
Collection中国科学院过程工程研究所
Corresponding AuthorXing, Jianmin
Affiliation1.Chinese Acad Sci, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Coll Chem Engn, 19 A Yuquan Rd, Beijing 100049, Peoples R China
3.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt
4.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China
5.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China
6.Suez Canal Univ, Fac Vet Med, Dept Virol, Ismailia 41522, Egypt
7.Chinese Acad Sci, Guangdong Prov Key Lab Synthet Genom, Shenzhen Key Lab Synthet Genom,Shenzhen Inst Adv, CAS Key Lab Quantitat Engn Biol,Shenzhen Inst Syn, Shenzhen 518055, Peoples R China
Recommended Citation
GB/T 7714
Sharshar, Moustafa Mohamed,Samak, Nadia Abdrabou,Ambreen, Sadaf,et al. Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus[J]. BIORESOURCE TECHNOLOGY,2020,317:11.
APA Sharshar, Moustafa Mohamed.,Samak, Nadia Abdrabou.,Ambreen, Sadaf.,Hao, Xuemi.,Mu, Tingzhen.,...&Xing, Jianmin.(2020).Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus.BIORESOURCE TECHNOLOGY,317,11.
MLA Sharshar, Moustafa Mohamed,et al."Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus".BIORESOURCE TECHNOLOGY 317(2020):11.
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