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Improvement of D-lactic acid production at low pH through expressing acid-resistant gene IoGAS1 in engineered Saccharomyces cerevisiae
Zhong, Wei1,2; Yang, Maohua2; Hao, Xuemi2,3; Sharshar, Moustafa Mohamed2,3; Wang, Qinhong4; Xing, Jianmin2,3
2020-11-02
Source PublicationJOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
ISSN0268-2575
Pages11
AbstractBACKGROUND Blending d-lactic acid (d-LA) with l-lactic acid can significantly improve the thermostability of polylactic (PLA). Although microbial production of d-LA under acidic conditions is beneficial for the reduction of production costs, the yield is low due to the acidic toxicity of the source strains. Herein, an Issatchenkia orientalis glycosylphosphatidylinositol-anchored protein IoGas1, which is required for resistance to low pH and salt stress, was expressed in the YIP-J-C-D-A1 yeast strain. This strain was integrated with Escherichia coli d-lactate dehydrogenase gene and several attenuated key pathway genes, including pyruvate decarboxylases (PDC1, PDC6), JEN1 (a monocarboxylate transporter), d-lactate dehydrogenase1 (DLD1), l-lactate cytochrome-c oxidoreductase (CYB2) and alcohol dehydrogenase 1(ADH1). RESULTS The results revealed that the production of d-LA by the modified strain YIP-I-J-C-D-A1 was remarkably improved and reached 85.3 g L-1 d-LA, with a yield of 0.71 g g(-1) and a productivity of 1.20 g L h(-1) in batch-fed fermentation. The d-LA production of the YIP-I-J-C-D-A1 strain (CGMCC2.5785) was further improved by attenuating the ethanol and glycerol pathways. The resulting strain YIP-A15G12 (CGMCC2.5803) produced 92.0 g L-1 d-LA with a yield of 0.70 g g(-1) and a productivity of 1.21 g L h(-1) in batch-fed fermentation at a final pH of 3.58. CONCLUSION Taken together, the expression of the acid-resistant gene IoGAS1 in a modified yeast strain can significantly improve the efficiency of producing d-LA at low pH, which may prove beneficial for the industrial production of the biodegradable material, PLA.
Keywordd‐ lactic acid acid‐ resistant gene IoGAS1 rational metabolic engineering Saccharomyces cerevisiae
DOI10.1002/jctb.6587
Language英语
WOS KeywordACETIC-ACID ; EFFICIENT PRODUCTION ; YEAST ; FERMENTATION ; TOLERANCE ; DEHYDROGENASE ; PATHWAY ; PROTEIN ; GROWTH ; OVEREXPRESSION
Funding ProjectNational Natural Science Foundation of China[21878307] ; National Natural Science Foundation of China[31800030] ; National Natural Science Foundation of China[31872633] ; Industrial Biotechnology Program of Tianjin Municipal Science and Technology Commission[14ZCZDSY00066]
WOS Research AreaBiotechnology & Applied Microbiology ; Chemistry ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Environmental ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China ; Industrial Biotechnology Program of Tianjin Municipal Science and Technology Commission
WOS IDWOS:000583406600001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42500
Collection中国科学院过程工程研究所
Corresponding AuthorWang, Qinhong; Xing, Jianmin
Affiliation1.Chinese Acad Sci, Shenzhen Key Lab Synthet Genom, Guangdong Prov Key Lab Synthet Genom,Shenzhen Ins, CAS Key Lab Quantitat Engn Biol,Shenzhen Inst Syn, Shenzhen, Peoples R China
2.Chinese Acad Sci, CAS Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Coll Chem Engn, Beijing, Peoples R China
4.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Wei,Yang, Maohua,Hao, Xuemi,et al. Improvement of D-lactic acid production at low pH through expressing acid-resistant gene IoGAS1 in engineered Saccharomyces cerevisiae[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2020:11.
APA Zhong, Wei,Yang, Maohua,Hao, Xuemi,Sharshar, Moustafa Mohamed,Wang, Qinhong,&Xing, Jianmin.(2020).Improvement of D-lactic acid production at low pH through expressing acid-resistant gene IoGAS1 in engineered Saccharomyces cerevisiae.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,11.
MLA Zhong, Wei,et al."Improvement of D-lactic acid production at low pH through expressing acid-resistant gene IoGAS1 in engineered Saccharomyces cerevisiae".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2020):11.
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