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| Preparation and evaluation of amphipathic lipopeptide-loaded PLGA microspheres as sustained-release system for AIDS prevention | |
Jin, Huijuan1,2; Chong, Huihui3; Zhu, Yuanmei3; Zhang, Mengqiu1,4; Li, Xun1,2; Bazybek, Nardana1,2; Wei, Yi1 ; Gong, Fangling1; He, Yuxian3; Ma, Guanghui1,2
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| 2020-11-01 | |
| Source Publication | ENGINEERING IN LIFE SCIENCES
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| ISSN | 1618-0240 |
| Volume | 20Issue:11Pages:476-484 |
| Abstract | At present, AIDS drugs are typical inhibitors that cannot achieve permanent effects. Therefore, the research of blocking HIV infection is essential. Especially for people in the high-risk environment, long-term prevention is important, because HIV can easily infect cells once the drug is interrupted. However, there is still no long-acting AIDS prevention drug approved. Hence, the purpose of this study is to prepare a fusion inhibitor loaded poly(d, l-lactic-co-glycolic acid) (PLGA) microspheres as a sustained-release system for long-term AIDS prevention. As the HIV membrane fusion inhibitor (LP-98) used in this research is amphiphilic lipopeptide, W-1/O/W-2 double-emulsion method was chosen, and premix membrane emulsification technique was used for controlling the uniformity of particle size. Several process parameters that can impact drug loading efficiency were summarized: the concentration of LP-98 and PLGA, and the preparation condition of primary emulsion. Finally, the microspheres with high loading efficiency (>8%) and encapsulation efficiency (>90%) were successfully prepared under optimum conditions. Pharmacokinetic studies showed that LP-98-loaded microspheres were capable to continuously release for 24 days in rats. This research can promote the application of sustained-release microspheres in AIDS prevention, and the embedding technique used in this study can also provide references for the loading of other amphipathic drugs. |
| Keyword | AIDS prevention amphipathic fusion inhibitor PLGA microspheres sustained‐ release |
| DOI | 10.1002/elsc.202000026 |
| Language | 英语 |
| WOS Keyword | NARROW SIZE DISTRIBUTION ; PREEXPOSURE PROPHYLAXIS ; DRUG-DELIVERY ; HIV ; ENCAPSULATION ; FORMULATION ; INHIBITION ; STRATEGIES ; TARGETS ; CANCER |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000586056400005 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/42573 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Wei, Yi; Gong, Fangling |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 Bei Er Jie, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing, Peoples R China 3.Chinese Acad Med Sci & Peking Union Med Coll, Inst Pathogen Biol, MOH Key Lab Syst Biol Pathogens, Beijing, Peoples R China 4.Wuhan Inst Technol, Wuhan, Peoples R China |
| Recommended Citation GB/T 7714 | Jin, Huijuan,Chong, Huihui,Zhu, Yuanmei,et al. Preparation and evaluation of amphipathic lipopeptide-loaded PLGA microspheres as sustained-release system for AIDS prevention[J]. ENGINEERING IN LIFE SCIENCES,2020,20(11):476-484. |
| APA | Jin, Huijuan.,Chong, Huihui.,Zhu, Yuanmei.,Zhang, Mengqiu.,Li, Xun.,...&Ma, Guanghui.(2020).Preparation and evaluation of amphipathic lipopeptide-loaded PLGA microspheres as sustained-release system for AIDS prevention.ENGINEERING IN LIFE SCIENCES,20(11),476-484. |
| MLA | Jin, Huijuan,et al."Preparation and evaluation of amphipathic lipopeptide-loaded PLGA microspheres as sustained-release system for AIDS prevention".ENGINEERING IN LIFE SCIENCES 20.11(2020):476-484. |
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