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Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System
Gong, Mengge1,2; Li, Tiechuan3; Wu, Lina2; Zhang, Zhenxing4; Ren, Lishi2; Duan, Xuexin; Cao, Hongzhi5; Pei, Meishan1; Li, Jian-Jun2; Du, Yuguang2
2020-11-01
Source PublicationCATALYSTS
Volume10Issue:11Pages:12
Abstract6 '/3 '-Sialylated N-acetyllactosamine (6 '/3 '-SLN) is important for discrimination of the source (human or avian) of influenza virus strains. Biotinylated oligosaccharides have been widely used for analysis and quick detection. The development of efficient strategies to synthesize biotin-tagged 6 '/3 '-SLN have become necessary. Effective mixing is essential for enzymatic solid-phase oligosaccharide synthesis (SPOS). In the current study, newly developed technology ultrahigh-frequency-acoustofluidics (UHFA), which can provide a powerful source for efficient microfluidic mixing, solid-phase oligosaccharide synthesis and one-pot multienzyme (OPME) system, were used to develop a new strategy for oligosaccharide synthesis. Firstly, biotinylated N-acetylglucosamine was designed and chemically synthesized through traditional approaches. Secondly, biotinylated 6 '- and 3 '-sialyl-N-acetylglucosamines were prepared in solution through two sequential OPME modules in with a yield of similar to 95%. Thirdly, 6 '-SLN was also prepared through UHFA-based enzymatic solid-phase synthesis on magnetic beads with a yield of 64.4%. The current strategy would be potentially used for synthesis of functional oligosaccharides.
Keywordbiotinylated 6 '/3 '-sialyl-N-acetylglucosamine one-pot multienzyme solid-phase oligosaccharide synthesis influenza viruses ultrahigh-frequency-acoustofluidics
DOI10.3390/catal10111347
Language英语
WOS KeywordEFFICIENT CHEMOENZYMATIC SYNTHESIS ; ACID ; OLIGOSACCHARIDE ; TETRASACCHARIDE ; STREPTAVIDIN ; AFFINITY ; GLYCANS ; CANCER ; ROLES ; CELLS
Funding ProjectNational Natural Science Foundation of China[21877114]
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000593655800001
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42730
Collection中国科学院过程工程研究所
Corresponding AuthorPei, Meishan; Li, Jian-Jun
Affiliation1.Univ Jinan, Sch Chem & Chem Engn, Jinan 250022, Peoples R China
2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Natl Engn Res Ctr Biotechnol Beijing, PLA,Inst Proc Engn,Key Lab Biopharmaceut Prod & F, Beijing 100190, Peoples R China
3.Tianjin Univ, State Key Lab Precis Measuring Technol & Instrume, Tianjin 300072, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China
5.Shandong Univ, State Key Lab Microbial Technol, Natl Glycoengn Res Ctr, Qingdao 266237, Peoples R China
Recommended Citation
GB/T 7714
Gong, Mengge,Li, Tiechuan,Wu, Lina,et al. Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System[J]. CATALYSTS,2020,10(11):12.
APA Gong, Mengge.,Li, Tiechuan.,Wu, Lina.,Zhang, Zhenxing.,Ren, Lishi.,...&Du, Yuguang.(2020).Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System.CATALYSTS,10(11),12.
MLA Gong, Mengge,et al."Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System".CATALYSTS 10.11(2020):12.
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