Knowledge Management System Of Institute of process engineering,CAS
| Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix | |
| Li, Ke1,2; Cao, Y-x1; Jiao, S-m2; Du, G-h3; Du, Y-g2; Qin, X-m1 | |
| 2020-11-24 | |
| Source Publication | FRONTIERS IN PHARMACOLOGY
 |
| ISSN | 1663-9812 |
| Volume | 11Pages:18 |
| Abstract | Saccharides are the most abundant substance with the strongest immunological activity in Astragali Radix (AR). However, systematic structure study and immunoactivity screening of polysaccharides with different molecular weights (Mw) in AR have yet to be conducted. In this study, Astragalus polysaccharides (APSs) were divided into three fragments of different Mw values, >2,000 kDa (APS-?), about 10 kDa (APS-II), and about 300 Da (APS-I), by using ultrafiltration for the first time. The structural differences of the three products were determined on the basis of monosaccharide composition, FT-IR spectrum, linkage analysis, and nuclear magnetic resonance analysis. Cellular immune activity experiments in vitro and cyclophosphamide immunosuppression animal model experiments in vivo for nonspecific and specific immunoactivity screening were applied to identify the most immunogenic fragment in APSs. Linkage analysis results showed that APS-?, APS-II, and APS-III have different attachment sites of monosaccharide residues. Immune screening experiments indicated that the Mw of the APSs influenced their activity, and APS-II had the strongest immunoenhancing activity among the products. This research may serve as a reference for further study on APSs with different structures and immune activities, and as a guidance for the quality control of APSs and the development of new APS products. |
| Keyword | Astragalus polysaccharide different molecular weight immunomodulatory activity nonspecific and specific structure characterization |
| DOI | 10.3389/fphar.2020.582091 |
| Language | 英语 |
| WOS Keyword | ANTIOXIDANT ACTIVITY |
| WOS Research Area | Pharmacology & Pharmacy |
| WOS Subject | Pharmacology & Pharmacy |
| WOS ID | WOS:000596267100001 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/42804 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Du, Y-g; Qin, X-m |
| Affiliation | 1.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China 3.Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Ke,Cao, Y-x,Jiao, S-m,et al. Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix[J]. FRONTIERS IN PHARMACOLOGY,2020,11:18. |
| APA | Li, Ke,Cao, Y-x,Jiao, S-m,Du, G-h,Du, Y-g,&Qin, X-m.(2020).Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix.FRONTIERS IN PHARMACOLOGY,11,18. |
| MLA | Li, Ke,et al."Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix".FRONTIERS IN PHARMACOLOGY 11(2020):18. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment