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Advances in bio-nylon 5X: discovery of new lysine decarboxylases for the high-level production of cadaverine
Xue, Yaju1; Zhao, Yongliang1; Ji, Xiuling1; Yao, Jiahao1; Busk, Peter Kamp3; Lange, Lene4; Huang, Yuhong1,2,5,6; Zhang, Suojiang1,2
2020-12-21
Source PublicationGREEN CHEMISTRY
ISSN1463-9262
Volume22Issue:24Pages:8656-8668
Abstract

Cadaverine (1,5-pentanediamine) is the most important precursor for nylon PA5X, which has an extremely competitive market due to the high consumption of engineered plastics and fibers. The key enzyme lysine decarboxylase is in desperate need for industrial bio-based cadaverine production. In this study, new lysine decarboxylases have been mined by peptide pattern recognition for the high-level production of cadaverine. The predicted enzymes were expressed in E. coli and analyzed as whole-cell biocatalysts. Two outstanding recombinant enzymes from Edwardsiella tarda and Aeromonas sp. (LdcEt and LdcAer, respectively), were further purified and characterized. The optimal pH and temperature for LdcEt and LdcAer were pH 7, 55 degrees C and pH 6, 50 degrees C, respectively. These two enzymes were stable over the pH range of 5-7 during 24 h incubation. Both of them still had activity after being incubated at pH 8. LdcEt and LdcAer also have good thermostability with a half-life of 14.5 h and 20.3 h at 60 degrees C, respectively. The kinetic analysis showed that they have high catalytic efficiency as the k(cat)/K-m (LdcEt: 243.28 s(-1) mM(-1); LdcAer: 266.86 s(-1) mM(-1)) was the highest when compared for all the related studies. The whole cell conversion by LdcEt with 0.1% (v/v) Triton X-100 can convert 100% 2 M l-lysine HCl to cadaverine in 2 h and the cadaverine productivity was high up to 103.47 +/- 4.37 g L-1 h(-1). The in vitro studies further found that unpurified cell-lysates of LdcEt had relatively higher activity compared to the whole cell conversion. Meanwhile, 0.4 mg mL(-1) purified LdcEt and LdcAer can efficiently produce 165.96 +/- 1.41 g L-1 and 155.84 +/- 4.63 g L-1 cadaverine only in 0.5 h, respectively. The high specific activity, pH, thermo-stability, and catalytic efficiency in vivo and in vitro, combined with simultaneous cell treatment with Triton X-100 and the bioconversion process, provide LdcEt with great potential in the economic and efficient production of cadaverine at the industrial scale.

DOI10.1039/d0gc03100c
Language英语
WOS KeywordEscherichia-coli ; Enzymes ; Gene ; Ph
Funding ProjectInnovation Academy for Green Manufacture, CAS[IAGM2020C19] ; Henan Key Research and Development Project[202102210046] ; Hebei Natural Science Foundation[B2020103010] ; Beijing Nova Program of Science and Technology[Z201100006820141] ; CAS Pioneer Hundred Program
WOS Research AreaChemistry ; Science & Technology - Other Topics
WOS SubjectChemistry, Multidisciplinary ; Green & Sustainable Science & Technology
Funding OrganizationInnovation Academy for Green Manufacture, CAS ; Henan Key Research and Development Project ; Hebei Natural Science Foundation ; Beijing Nova Program of Science and Technology ; CAS Pioneer Hundred Program
WOS IDWOS:000598242900010
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42820
Collection中国科学院过程工程研究所
Corresponding AuthorHuang, Yuhong; Zhang, Suojiang
Affiliation1.Chinese Acad Sci, Beijing Key Lab Ion Liquids Clean Proc, State Key Lab Multiphase Complex Syst, CAS Key Lab Green Proc & Engn,Inst Proc Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Innovat Acad Green Mfg, Beijing 100190, Peoples R China
3.Roskilde Univ, Dept Sci & Environm, Univ Vej 1, DK-4000 Roskilde, Denmark
4.BioEcon Res & Advisory, DK-2500 Valby, Denmark
5.Zhengzhou Zhongke Emerging Ind Technol Res Inst, Zhengzhou 450000, Henan, Peoples R China
6.Zhongke Langfang Inst Proc Engn, Langfang 065001, Peoples R China
Recommended Citation
GB/T 7714
Xue, Yaju,Zhao, Yongliang,Ji, Xiuling,et al. Advances in bio-nylon 5X: discovery of new lysine decarboxylases for the high-level production of cadaverine[J]. GREEN CHEMISTRY,2020,22(24):8656-8668.
APA Xue, Yaju.,Zhao, Yongliang.,Ji, Xiuling.,Yao, Jiahao.,Busk, Peter Kamp.,...&Zhang, Suojiang.(2020).Advances in bio-nylon 5X: discovery of new lysine decarboxylases for the high-level production of cadaverine.GREEN CHEMISTRY,22(24),8656-8668.
MLA Xue, Yaju,et al."Advances in bio-nylon 5X: discovery of new lysine decarboxylases for the high-level production of cadaverine".GREEN CHEMISTRY 22.24(2020):8656-8668.
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