CAS OpenIR
Detection of Type I and III collagen in porcine acellular matrix using HPLC-MS
Zhang, Yang1,2; Chen, Yi3; Zhao, Bo3; Gao, Jianping1; Xia, Leilei3; Xing, Fangyu1; Kong, Yingjun1; Li, Yongchao2; Zhang, Guifeng1
2020-12-01
Source PublicationREGENERATIVE BIOMATERIALS
ISSN2056-3418
Volume7Issue:6Pages:577-582
AbstractAcellular matrix (ACM) has been widely used as a biomaterial. As the main component of ACM, collagen type and content show influence on the material properties. In this research, the collagen in ACM from different tissues of pig were determined by detection of marker peptides. The marker peptides of Type I and III collagen were identified from the digested collagen standards using ions trap mass spectrometry (LCQ). The relationship between the abundance of marker peptide and collagen concentration was established using triple quadrupole mass spectrometer (TSQ). The contents of Type I and III collagen in ACM from different tissues were determined. The method was further verified by hydroxyproline determination. The results showed that, the sum of Type I and III collagen contents in the ACM from small intestinal submucosa, dermis and Achilles tendon of pig were about 87.59, 81.41 and 61.13%, respectively, which were close to the total collagen contents in these tissues. The results proved that this method could quantitatively detect the collagen with different types in the ACM of various tissues.
KeywordHPLC-MS marker peptides acellular matrix collagen
DOI10.1093/rb/rbaa032
Language英语
WOS KeywordRECOVERY ; SCAFFOLD ; REPAIR ; TISSUE
Funding ProjectNational Key Technology R&D Programs of China[2018YFA0108200] ; National Key Technology R&D Programs of China[2018YFC1106400] ; Beijing Municipal Science and Technology Commission[Z181100006218013] ; Beijing Municipal Science and Technology Commission[XX2018008] ; Guangzhou People's Livelihood Science and Technology Project of China[201803010086] ; Key scientific research project plan of colleges and universities in Henan Province[17A530002]
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
Funding OrganizationNational Key Technology R&D Programs of China ; Beijing Municipal Science and Technology Commission ; Guangzhou People's Livelihood Science and Technology Project of China ; Key scientific research project plan of colleges and universities in Henan Province
WOS IDWOS:000607038800005
PublisherOXFORD UNIV PRESS
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/43204
Collection中国科学院过程工程研究所
Corresponding AuthorLi, Yongchao; Zhang, Guifeng
Affiliation1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Henan Inst Sci & Technol, Sch Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China
3.Beijing Biosis Healing Biol Technol Co Ltd, Beijing 100026, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yang,Chen, Yi,Zhao, Bo,et al. Detection of Type I and III collagen in porcine acellular matrix using HPLC-MS[J]. REGENERATIVE BIOMATERIALS,2020,7(6):577-582.
APA Zhang, Yang.,Chen, Yi.,Zhao, Bo.,Gao, Jianping.,Xia, Leilei.,...&Zhang, Guifeng.(2020).Detection of Type I and III collagen in porcine acellular matrix using HPLC-MS.REGENERATIVE BIOMATERIALS,7(6),577-582.
MLA Zhang, Yang,et al."Detection of Type I and III collagen in porcine acellular matrix using HPLC-MS".REGENERATIVE BIOMATERIALS 7.6(2020):577-582.
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