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| Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms | |
| Kang, Dingrong1,4; Huang, Yuhong2,6; Nesme, Joseph1; Herschend, Jakob1; Jacquiod, Samuel1,5; Kot, Witold3; Hansen, Lars Hestbjerg3; Lange, Lene2,7; Sorensen, Soren J.1 | |
| 2021-03-20 | |
| Source Publication | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| ISSN | 0048-9697 |
| Volume | 761Pages:9 |
| Abstract | Keratin is an insoluble fibrous protein from natural environments, which can be recycled to value-added products by keratinolytic microorganisms. A microbial consortium with efficient keratinolytic activity was previously enriched from soil, but the genetic basis behind its remarkable degradation properties was not investigated yet. To identify the metabolic pathways involved in keratinolysis and clarify the observed synergy among community members, shotgun metagenomic sequencing was performed to reconstruct metagenome-assembled genomes. More than 90% genera of the enriched bacterial consortium were affiliated to Chryseobacterium, Stenotrophomonas, and Pseudomonas. Metabolic potential and putative keratinases were predicted from the metagenomic annotation, providing the genetic basis of keratin degradation. Furthermore, metabolic pathways associated with keratinolytic processes such as amino acid metabolism, disulfide reduction and urea cycle were investigated from seven high-quality metagenome-assembled genomes, revealing the potential metabolic cooperation related to keratin degradation. This knowledge deepens the understanding ofmicrobial keratinolytic mechanisms at play in a complex community, pinpointing the significance of synergistic interactions, which could be further used to optimize industrial keratin degradation processes. (C) 2020 Elsevier B.V. All rights reserved. |
| Keyword | Microbial community Keratinases Metagenome-assembled genomes Metabolic pathways Metabolic cooperation |
| DOI | 10.1016/j.scitotenv.2020.143281 |
| Language | 英语 |
| WOS Keyword | ENZYMES ; EXPRESSION ; STABILITY ; SEQUENCE ; GENOMES ; WASTE ; CLASSIFICATION ; OPTIMIZATION ; DEGRADATION ; QUALITY |
| Funding Project | Danish Innovation Fund[1308-00015B] ; Chinese Scholarship Council (CSC) Scholarship Program |
| WOS Research Area | Environmental Sciences & Ecology |
| WOS Subject | Environmental Sciences |
| Funding Organization | Danish Innovation Fund ; Chinese Scholarship Council (CSC) Scholarship Program |
| WOS ID | WOS:000607780900084 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/43245 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Sorensen, Soren J. |
| Affiliation | 1.Univ Copenhagen, Dept Biol, Sect Microbiol, Copenhagen, Denmark 2.Tech Univ Denmark, Dept Biotechnol & Biomed, Bldg 224, DK-2800 Lyngby, Denmark 3.Univ Copenhagen, Dept Plant & Environm Sci, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark 4.Univ Liege, TERRA Res & Teaching Ctr, Microbial Proc & Interact MiPI, Gembloux Agrobio Tech, Gembloux, Belgium 5.Univ Bourgogne Franche Comte, INRA, AgroSup Dijon, Agroecol, Besancon, France 6.Chinese Acad Sci, Inst Proc Engn, Beijing Key Lab Ion Liquids Clean Proc, Key Lab Green Proc & Engn,State Key Lab Multiphas, Beijing 100190, Peoples R China 7.Bioecon Res & Advisory, Karensgade 5, DK-2500 Valby, Denmark |
| Recommended Citation GB/T 7714 | Kang, Dingrong,Huang, Yuhong,Nesme, Joseph,et al. Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,761:9. |
| APA | Kang, Dingrong.,Huang, Yuhong.,Nesme, Joseph.,Herschend, Jakob.,Jacquiod, Samuel.,...&Sorensen, Soren J..(2021).Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms.SCIENCE OF THE TOTAL ENVIRONMENT,761,9. |
| MLA | Kang, Dingrong,et al."Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms".SCIENCE OF THE TOTAL ENVIRONMENT 761(2021):9. |
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