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On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines
Song, Yanmin1,2; Yang, Yanli1; Lin, Xuan1; Zhao, Qizu3; Li, Zhengjun1; Ma, Guanghui1; Su, Zhiguo1; Zhang, Songping1
2021-01-25
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1637Pages:8
AbstractAccurate quantification of effective antigens of different serotypes is crucial for quality control of multivalent vaccines but challenging. A simple and rapid capillary zone electrophoresis (CZE) method was developed for on-line separation and quantification of foot-and-mouth disease virus (FMDV) antigens in monovalent and bivalent FMDV vaccines. The FMDV peak identity in CZE was demonstrated by the study of FMDV dissociation combined with high performance size exclusion chromatography (HPSEC) analysis. After optimizing CZE conditions including UV detecting wavelength, injection volume, and separation voltage, both serotype A and O FMDV showed good reproducibility (RSD <5%) and linear responses (R-2 =0.999) between the peak area and FMDV content in the concentration range of 15-400 mu g/mL. The two serotypes of FMDV with similar size had different migration time in CZE according to their different zeta potential, which allows them to be separated and quantified, with accuracy of <10% relative error. CZE was then successfully applied for antigen quantification of commercial O monovalent and A/O bivalent FMDV vaccines. Compared with HPSEC, CZE was not only able to quantify each serotype of FMDV, but also free from interference of nucleic acids impurities. In summary, the CZE can be a simple, rapid, and reliable tool for quality control of monovalent and bivalent FMDV vaccines. The CZE method can also be further extended to the quality control of other multivalent virus and virus like particle vaccines. (C) 2020 Elsevier B.V. All rights reserved.
KeywordCapillary zone electrophoresis FMDV Quantitation Serotyping Quality control
DOI10.1016/j.chroma.2020.461834
Language英语
Funding ProjectNational Natural Science Foundation of China[21808226] ; National Natural Science Foundation of China[31970872] ; National Natural Science Foundation of China[21821005]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000616104700001
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/43466
Collection中国科学院过程工程研究所
Corresponding AuthorYang, Yanli; Zhang, Songping
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
3.China Inst Vet Drug Control, Beijing 100081, Peoples R China
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Song, Yanmin,Yang, Yanli,Lin, Xuan,et al. On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines[J]. JOURNAL OF CHROMATOGRAPHY A,2021,1637:8.
APA Song, Yanmin.,Yang, Yanli.,Lin, Xuan.,Zhao, Qizu.,Li, Zhengjun.,...&Zhang, Songping.(2021).On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines.JOURNAL OF CHROMATOGRAPHY A,1637,8.
MLA Song, Yanmin,et al."On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines".JOURNAL OF CHROMATOGRAPHY A 1637(2021):8.
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