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| Performance of agarose and gigaporous chromatographic media as function of pore-to-adsorbate size ratio over wide span from ovalbumin to virus like particles | |
| Yang, Yanli1; Yu, Mengran1,2; Ma, Guanghui1; Su, Zhiguo1; Zhang, Songping1 | |
| 2021-02-08 | |
| Source Publication | JOURNAL OF CHROMATOGRAPHY A
 |
| ISSN | 0021-9673 |
| Volume | 1638Pages:12 |
| Abstract | Two commercially available agarose ion exchange media, DEAE-Capto and DEAE-Sepharose FF (DEAE-FF), and two gigaporous media DEAE -AP-120 nm and DEAE-AP-280 nm were evaluated for their applicability in adsorption of five proteins with large span of radius ranges from 2.9 nm to 14.1 nm, which include ovalbumin, bovine serum albumin (BSA), haptoglobin, thyroglobulin and hepatitis B surface antigen (HBsAg) virus like particle. The average pore radius of the four media was determined to be 6.9 nm, 18.5 nm, 59.4 nm and 139.3 nm, respectively, which was obtained by log normal distribution for DEAE-Capto and DEAE-FF and by bimodal Gaussian distribution for the two DEAE-AP media. The performance of these four media including phase ratio, static and dynamic binding capacity, and transport properties for the adsorption of these five model proteins as function of pore-to-adsorbate size ratio were investigated and compared. The best ratio of pore-to-adsorbate size was found dependent on the protein size. For protein with radius from 2.9 nm (ovalbumin) to 5.4 nm (BSA), the agarose media was superior to gigaporous media. Both the static and dynamic adsorption capacities reduced with the increase of pore size, and the highest values were obtained at the smallest pore-to-adsorbate size of about 2 times in this study, although the highest accessible surface area was obtained at pore-to-adsorbate size ratio about 16 to 20. For proteins with radius of 5.4 nm or larger than that, their adsorption capacities decreased firstly and then increased with the increase of ratio of pore-to-adsorbate size, and the highest values were obtained on the gigaporous media DEAE-AP-280 nm, which could provide faster diffusivity and larger accessible surface area. However, protein with radius of 14.1 nm (HBsAg) had much lower capacities compared to other proteins at the same pore-to-adsorbate size ratio, implying large protein needs greater pore-to-adsorbate size ratio to achieve a satisfactory capacity. For all the five tested proteins, the DEAE-Capto media having the smallest pore radius and branched dextran chains, was found superior to DEAE-FF in terms of both higher adsorption capacities and uptake kinetics, which suggested that the "chain delivery effect" took place on proteins over large size span from ovalbumin to HBsAg, though the effect on the larger proteins was much less significant than that on the smaller ones. Results from the present work provided more information on how do the relationships of pore size of chromatography media and adsorbate size interactively affect the chromatography behaviors, thus will provide general guidance for selection of suitable adsorbent for biologics of a given size. (C) 2021 Elsevier B.V. All rights reserved. |
| Keyword | Pore size Protein size Ion-exchange media Ratio of pore-to-adsorbate size Adsorption capacity Adsorption kinetics |
| DOI | 10.1016/j.chroma.2021.461879 |
| Language | 英语 |
| WOS Keyword | PROTEIN ; ADSORPTION ; DISTRIBUTIONS |
| Funding Project | National Natural Science Foundation of China[21821005] ; National Natural Science Foundation of China[31970872] ; National Natural Science Foundation of China[21808226] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemical Research Methods ; Chemistry, Analytical |
| Funding Organization | National Natural Science Foundation of China |
| WOS ID | WOS:000618077200011 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/43495 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Su, Zhiguo; Zhang, Songping |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Global Life Sci Technol Shanghai Co Ltd, Shanghai 201203, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Yanli,Yu, Mengran,Ma, Guanghui,et al. Performance of agarose and gigaporous chromatographic media as function of pore-to-adsorbate size ratio over wide span from ovalbumin to virus like particles[J]. JOURNAL OF CHROMATOGRAPHY A,2021,1638:12. |
| APA | Yang, Yanli,Yu, Mengran,Ma, Guanghui,Su, Zhiguo,&Zhang, Songping.(2021).Performance of agarose and gigaporous chromatographic media as function of pore-to-adsorbate size ratio over wide span from ovalbumin to virus like particles.JOURNAL OF CHROMATOGRAPHY A,1638,12. |
| MLA | Yang, Yanli,et al."Performance of agarose and gigaporous chromatographic media as function of pore-to-adsorbate size ratio over wide span from ovalbumin to virus like particles".JOURNAL OF CHROMATOGRAPHY A 1638(2021):12. |
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