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Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli
Wang, Dan1,2; Li, Qiang1,2; Li, Wangliang1; Xing, Jianmin1; Su, Zhiguo1
2009-12-01
Source PublicationENZYME AND MICROBIAL TECHNOLOGY
ISSN0141-0229
Volume45Issue:6-7Pages:491-497
AbstractSuccinate fermentation was investigated in Escherichia coli strains overexpressing cyanobacterium Anabaena sp. 7120 ecaA gene encoding carbonic anhydrase (CA). In strain BL21 (DE3) bearing ecaA, the activity of CA was 21.8 U mg(-1) protein, whereas non-detectable CA activity was observed in the control strain. Meanwhile. the activity of phosphoenolpyruvate carboxylase (PEPC) increased from 0 2 U mg(-1) protein to 1.13 U mg(-1) protein. The recombinant bearing ecaA reached a succinate yield of 0.39 mol mol(-1) in glucose at the end of the fermentation. It was 21-fold higher than that of con trot strain which was Just 0 19 mol mol(-1) glucose EcaA gene was also introduced into E coli DC1515, which was deficient in glucose phosphotransferase. lactate dehydrogenase and pyruvate formate lyase succinate yield call be further increased to 1 26 mol mol(-1) glucose It could be concluded that the enhancement of the supply of HCO3- in vivo by ecaA overexpression is all effective strategy for the Improvement of succinate production in E. Coli. (C) 2009 Elsevier Inc All rights reserved.
KeywordSuccinate Escherichia Coli Carbonic Anhydrase (Ca) Bicarbonate Carbon Dioxide
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.enzmictec.2009.08.003
Indexed BySCI
Language英语
WOS KeywordPHOSPHOENOLPYRUVATE CARBOXYKINASE ; PYRUVATE-CARBOXYLASE ; ACID PRODUCTION ; MANNHEIMIA-SUCCINICIPRODUCENS ; LACTATE-DEHYDROGENASE ; MALIC ENZYME ; BOVINE RUMEN ; FERMENTATION ; EXPRESSION ; CO2
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000271260700013
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4370
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Dan,Li, Qiang,Li, Wangliang,et al. Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli[J]. ENZYME AND MICROBIAL TECHNOLOGY,2009,45(6-7):491-497.
APA Wang, Dan,Li, Qiang,Li, Wangliang,Xing, Jianmin,&Su, Zhiguo.(2009).Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli.ENZYME AND MICROBIAL TECHNOLOGY,45(6-7),491-497.
MLA Wang, Dan,et al."Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli".ENZYME AND MICROBIAL TECHNOLOGY 45.6-7(2009):491-497.
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