CAS OpenIR
胸腺素α1的DIC固相化学合成与鉴定
甘一如1; 黄永东1; 刘晋生1; 张雪竹1; 张贵峰2
2004
Source Publication化工学报
ISSN0438-1157
Volume55.0Issue:002Pages:305
AbstractThymosin α1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α1 was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the a-amino acid and N,N'-diisopropylcarbodiimide (DIC) as the coupling regent. The crude product was analyzed by reversed-phase high performance liquid chromatography, SDS-PAGE and liquid chromatography/mass spectroscopy. The synthesis yield was 84.89%. The analytical results indicated that the crude product contained much thymosin α1, and the purity was 50.3%. DIC solid-phase chemical synthesis of thymosin α1 was applicable, and the yield was improved greatly compared to other known methods.
Keyword胸腺素α1 固相多肽合成 二异丙基碳二亚胺(DIC) 反相高效液相色谱法 聚丙烯酰胺凝胶电泳 (SDS-PAGE)法 液相色谱/质谱(LC/MS)
Language英语
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/45043
Collection中国科学院过程工程研究所
Affiliation1.天津大学
2.中国科学院过程工程研究所
Recommended Citation
GB/T 7714
甘一如,黄永东,刘晋生,等. 胸腺素α1的DIC固相化学合成与鉴定[J]. 化工学报,2004,55.0(002):305.
APA 甘一如,黄永东,刘晋生,张雪竹,&张贵峰.(2004).胸腺素α1的DIC固相化学合成与鉴定.化工学报,55.0(002),305.
MLA 甘一如,et al."胸腺素α1的DIC固相化学合成与鉴定".化工学报 55.0.002(2004):305.
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