CAS OpenIR
牛关节软骨II型胶原的分离纯化与鉴定
李赛娜1; 康跻耀2; 邓平晔3; 高建萍2; 张贵锋2; 王明林1
2016
Source Publication过程工程学报
ISSN1009-606X
Volume16Issue:4Pages:654
Abstract将牛关节软骨用NaCl和盐酸胍预处理,在酸性条件下用胃蛋白酶降解杂蛋白,经盐析、透析和冷冻干燥后获得II型胶原,对其进行鉴定.结果表明,提取的II型胶原纯度为92.2%,得率为61.5%,单条链分子量为130 kDa,氨基酸组成中Gly含量超过30%, Pro与Hyp总含量超过20%,具有胶原特有的超分子结构,中性条件下变性温度为43.68℃,符合II型胶原特性.
KeywordII型胶原 分离 生物质谱 特征多肽
Language英语
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/46431
Collection中国科学院过程工程研究所
Affiliation1.山东农业大学
2.中国科学院过程工程研究所
3.北京市理化分析测试中心
Recommended Citation
GB/T 7714
李赛娜,康跻耀,邓平晔,等. 牛关节软骨II型胶原的分离纯化与鉴定[J]. 过程工程学报,2016,16(4):654.
APA 李赛娜,康跻耀,邓平晔,高建萍,张贵锋,&王明林.(2016).牛关节软骨II型胶原的分离纯化与鉴定.过程工程学报,16(4),654.
MLA 李赛娜,et al."牛关节软骨II型胶原的分离纯化与鉴定".过程工程学报 16.4(2016):654.
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