Shen Xiaofei1; Zeng Shaohua2; Wu Min2; Liu Chunzhao3; Wang Ying1
Source PublicationJournal of Chinese Pharmaceutical Sciences
AbstractProanthocyanidins (PAs) are a set of important phytochemical compounds in Lycium ruthenicum Murr. However, PA biosynthesis in L. ruthenicum remains unclear. In this study, leucoanthocyanidin reductase (LAR) and anthocyanidin reductases (ANR) involved in PA biosynthesis were retrieved from L. ruthenicum EST database. Polymerase chain reaction (PCR) results confirmed that LrLAR and LrANR encoded a protein composed of 333 and 338 amino acids, respectively. Phylogenetic analysis showed that LrLAR and LrANR were clustered with known LAR and ANR proteins of other plants, respectively. Quantitative reversetranscription PCR (qRT-PCR) results indicated that both genes expressed in a sharp increase manner from unripe stage to color break stage and were downregulated increasingly hereafter, and that both expression levels are extremely low in young leaves, mature leaves, stems, and roots compared with fruits. Phytochemical assay revealed that the content of total PA in fruit was higher than that in young leaves, mature leaves, stems, and roots, and that the total PA level was increased sharply and then relatively stable hereafter during fruit ripening. Our results lay a foundation for uncovering the PA biosynthesis and further engineering manipulation in L. ruthenicum.
Keyword花青素还原酶 无色花色素还原酶 黑果枸杞 原花青素
Document Type期刊论文
Recommended Citation
GB/T 7714
Shen Xiaofei,Zeng Shaohua,Wu Min,等. 黑果枸杞原花青素合成相关无色花色素还原酶基因和花青素还原酶基因的克隆及表达分析[J]. Journal of Chinese Pharmaceutical Sciences,2014,23(6):369.
APA Shen Xiaofei,Zeng Shaohua,Wu Min,Liu Chunzhao,&Wang Ying.(2014).黑果枸杞原花青素合成相关无色花色素还原酶基因和花青素还原酶基因的克隆及表达分析.Journal of Chinese Pharmaceutical Sciences,23(6),369.
MLA Shen Xiaofei,et al."黑果枸杞原花青素合成相关无色花色素还原酶基因和花青素还原酶基因的克隆及表达分析".Journal of Chinese Pharmaceutical Sciences 23.6(2014):369.
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