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肝素-琼脂糖凝胶6FF的制备及其在抗凝血酶III分离纯化中的应用
穆成华; 姚红娟; 马光辉; 连宾
2005
Source Publication过程工程学报
Issue05Pages:545-549
Abstract以自制琼脂糖凝胶6FF为基质,肝素为配基,利用还原胺化方法制备了肝素-琼脂糖凝胶6FF介质.考察了肝素偶联反应的影响因素,并对其进行了优化,得到肝素配基的含量为2.7mg/mL.用所制介质从人血浆中分离出抗凝血酶III,从血浆开始计算的抗凝血酶III活性回收率为31.76%,与商品Heparin-SepharoseCL-6B的分离效果相当.
Keyword亲和层析 肝素 抗凝血酶iii
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4738
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
穆成华,姚红娟,马光辉,等. 肝素-琼脂糖凝胶6FF的制备及其在抗凝血酶III分离纯化中的应用[J]. 过程工程学报,2005(05):545-549.
APA 穆成华,姚红娟,马光辉,&连宾.(2005).肝素-琼脂糖凝胶6FF的制备及其在抗凝血酶III分离纯化中的应用.过程工程学报(05),545-549.
MLA 穆成华,et al."肝素-琼脂糖凝胶6FF的制备及其在抗凝血酶III分离纯化中的应用".过程工程学报 .05(2005):545-549.
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