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扩张床吸附直接从牛血中快速分离牛血清白蛋白
路秀玲; 赵东旭; 金业涛; 苏志国
2005
Source Publication中国生化药物杂志
Issue01Pages:5-8
Abstract目的研究扩张床吸附技术直接从牛血中快速分离血浆蛋白。方法用葡萄糖等张液与牛血按4∶1混合,混合液的渗透压在2 80~30 0mOsmo范围内,与血浆的总渗透压相近;pH值降至6 .8,黏度从全血4 .7mPa·s降至1.0mPa·s以下,再用扩张床StreamlineDEAE对血浆蛋白进行吸附洗脱。结果扩张床洗脱液中单体牛血清白蛋白(BSA)的纯度接近70 % ,全血进料时血浆蛋白吸附容量为纯BSA进料的2 1.4 4 %。结论扩张床技术直接从未经固液分离的牛血血浆中分离BSA ,只经一步操作,实现了BSA的有效快速分离
Keyword扩张床吸附 牛血清白蛋白 分离
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4774
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
路秀玲,赵东旭,金业涛,等. 扩张床吸附直接从牛血中快速分离牛血清白蛋白[J]. 中国生化药物杂志,2005(01):5-8.
APA 路秀玲,赵东旭,金业涛,&苏志国.(2005).扩张床吸附直接从牛血中快速分离牛血清白蛋白.中国生化药物杂志(01),5-8.
MLA 路秀玲,et al."扩张床吸附直接从牛血中快速分离牛血清白蛋白".中国生化药物杂志 .01(2005):5-8.
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