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Energy-efficient butanol production by Clostridium acetobutylicum with histidine kinase knockouts to improve strain tolerance and process robustness
Du, Guangqing1,2; Zhu, Chao1,2; Xu, Mengmeng3; Wang, Lan4; Yang, Shang-Tian3; Xue, Chuang1,2
2021-03-07
Source PublicationGREEN CHEMISTRY
ISSN1463-9262
Volume23Issue:5Pages:2155-2168
AbstractUnder stress, Clostridium acetobutylicum sporulates and halts its metabolism, which limits its use in industrial acetone-butanol-ethanol (ABE) fermentation. It is challenging to manipulate the highly regulated sporulation program used by clostridia for survival. Four orphan histidine kinases (HKs) involved in the phosphorylation of Spo0A, a global regulator, were found to control sporulation, cell growth, and metabolism (acidogenesis and solventogenesis). Single knockout of cac3319 aborted sporulation at the initiation stage but enhanced butanol tolerance and production, whereas cac0437 knockout caused premature sporulation and inhibited the transition from acidogenesis to solventogenesis, resulting in early autolysis and low butanol production. No solvent was produced when both cac3319 and cac0903 were knocked out because the mutant lost the megaplasmid carrying solventogenesis genes. On the other hand, a mutant with double knockouts of cac3319 and cac0323 showed enhanced butanol production of >20 g L-1 by a repeated batch fermentation in a fibrous bed bioreactor, which enabled in situ product recovery via vapor stripping-vapor permeation to achieve the highest butanol production of 441.9 g L-1 ever reported. This study elucidated the regulatory roles of 4 HKs in controlling clostridial sporulation and solventogenesis with morphological, transcriptomics, and fermentation kinetics analyses, and demonstrated combinatory knockouts of HKs as a novel metabolic engineering approach to abort sporulation and enhance butanol production in ABE fermentation suitable for industrial application.
DOI10.1039/d0gc03993d
Language英语
Funding ProjectNational Natural Science Foundation of China (NSFC)[21878035] ; National Natural Science Foundation of China (NSFC)[21576045] ; Liaoning Revitalization Talents Program[XLYC1807269] ; National Key R&D Program of China[2018YFB1501703] ; Dalian Science and Technology Innovation Project[2018J12SN074] ; National Key Laboratory of Biochemical Engineering[2018KF-03] ; Fundamental Research Funds for the Central Universities[DUT19ZD213]
WOS Research AreaChemistry ; Science & Technology - Other Topics
WOS SubjectChemistry, Multidisciplinary ; Green & Sustainable Science & Technology
Funding OrganizationNational Natural Science Foundation of China (NSFC) ; Liaoning Revitalization Talents Program ; National Key R&D Program of China ; Dalian Science and Technology Innovation Project ; National Key Laboratory of Biochemical Engineering ; Fundamental Research Funds for the Central Universities
WOS IDWOS:000628913600027
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/48032
Collection中国科学院过程工程研究所
Corresponding AuthorXue, Chuang
Affiliation1.Dalian Univ Technol, Sch Bioengn, Engn Res Ctr Applicat & Transformat Synth Biol, Dalian 116024, Peoples R China
2.Dalian Univ Technol, Ningbo Inst, Ningbo 315016, Peoples R China
3.Ohio State Univ, Dept Chem & Biomol Engn, 151 West Woodruff Ave, Columbus, OH 43210 USA
4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 10090, Peoples R China
Recommended Citation
GB/T 7714
Du, Guangqing,Zhu, Chao,Xu, Mengmeng,et al. Energy-efficient butanol production by Clostridium acetobutylicum with histidine kinase knockouts to improve strain tolerance and process robustness[J]. GREEN CHEMISTRY,2021,23(5):2155-2168.
APA Du, Guangqing,Zhu, Chao,Xu, Mengmeng,Wang, Lan,Yang, Shang-Tian,&Xue, Chuang.(2021).Energy-efficient butanol production by Clostridium acetobutylicum with histidine kinase knockouts to improve strain tolerance and process robustness.GREEN CHEMISTRY,23(5),2155-2168.
MLA Du, Guangqing,et al."Energy-efficient butanol production by Clostridium acetobutylicum with histidine kinase knockouts to improve strain tolerance and process robustness".GREEN CHEMISTRY 23.5(2021):2155-2168.
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