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| Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres | |
Hao, Dongxia1 ; Zhang, Rongyue2; Ge, Jia1; Ye, Peili1; Song, Cui1; Zhu, Kai1; Na, Xiangming1; Huang, Yongdong1; Zhao, Lan1; Zhou, Weiqing1; Su, Zhiguo1; Ma, Guanghui1
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| 2021-03-15 | |
| Source Publication | JOURNAL OF CHROMATOGRAPHY A
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| ISSN | 0021-9673 |
| Volume | 1640Pages:10 |
| Abstract | Fast-throughput and cost reduction of current purification platforms are becoming increasing requests during antibody manufacture. The macroporous-matrix absorbents have presented extensive potentiality in improving operational throughput during purification of macromolecule. And meanwhile the peptide ligand has become a promising alternative to recombinant protein ligands for cost reduction of chromatographic purification. Therefore, here we designed a functionalized microspheres resin with both macroporous matrix of polymerized glycidyl methacrylate and ethylene glycol dimethacrylate (PGMA-EDMA) and peptide ligand of hexapeptide (FYEILH). In order to circumvent the steric effect of peptides and amplify the binding sites on macroporous matrix, the peptide ligand was coupled on a liner PGMA polymer brushes grafted on microspheres. Comparing to the conventional agarose-matrix resin and the general peptide-grafted microspheres, the functionalized microspheres presented excellent permeability and high capacity to rapid loading hIgG by maintaining a stable level of dynamic binding capacity at fast flow rate above 110 column volume per hour (cv/h) and very short residence time below 0.5 min. Such functionalized microspheres provide a facile and broadly applicable strategy to develop the attractive candidate for rapid and cost-reduced purification of antibody. (c) 2021 Elsevier B.V. All rights reserved. |
| Keyword | Antibody purification Peptide ligand Polymer brush Macroporous microspheres |
| DOI | 10.1016/j.chroma.2021.461948 |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[21676275] ; National Natural Science Foundation of China[22078334] ; National Natural Science Foundation of China[21821005] ; Beijing Natural Science Foundation[L160012] ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee[ZZB2019001] ; National Key Research and Development Program of China[2018YFC0311101] ; Beijing Natural Science FoundationHaidian Original Innovation Joint Fund[L192017] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemical Research Methods ; Chemistry, Analytical |
| Funding Organization | National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee ; National Key Research and Development Program of China ; Beijing Natural Science FoundationHaidian Original Innovation Joint Fund |
| WOS ID | WOS:000624599200011 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/48072 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Ma, Guanghui |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, POB 353, Beijing 100190, Peoples R China 2.Beijing Inst Petrochem Technol, Beijing 102617, Peoples R China |
| Recommended Citation GB/T 7714 | Hao, Dongxia,Zhang, Rongyue,Ge, Jia,et al. Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres[J]. JOURNAL OF CHROMATOGRAPHY A,2021,1640:10. |
| APA | Hao, Dongxia.,Zhang, Rongyue.,Ge, Jia.,Ye, Peili.,Song, Cui.,...&Ma, Guanghui.(2021).Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres.JOURNAL OF CHROMATOGRAPHY A,1640,10. |
| MLA | Hao, Dongxia,et al."Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres".JOURNAL OF CHROMATOGRAPHY A 1640(2021):10. |
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