CAS OpenIR
Insight of Synergistic Effect between CPP and Cargo on the Facilitation Mechanisms of R7-PTX Translocation: Experiments and Molecular Simulations
Wei, Yuping1,5; Zhang, Caiying1; Zhang, Man1,4; Niu, Qionghong1; Hui, Fengli1; Liu, Zi2,3; Xu, Xia2,3,5
2021-06-01
Source PublicationEUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
ISSN0928-0987
Volume161Pages:9
AbstractIn our previous study, a novel cell penetrating peptide (CPP) R7 (Arg-Arg-Arg-Arg-Arg-Trp-Trp, RRRRRWW) has been developed to help cellular internalization of paclitaxel (PTX) through the non-covalent interaction with CPP. However, the facilitation mechanism of R7 mediated PTX translocation is not clear. Here the uptake pathways of R7 and R7-PTX were investigated by in vitro test and molecular simulations. In vitro experiments reveal that both R7 and R7-PTX complex translocate through the direct translocation and clathrin mediated endocytosis and associate with the macropinocytosis pathway at high CPP concentration. The translocation of R7 (0.1 mM)-PTX complex further involves the lipid raft/caveolae mediated endocytosis. The simulation results show that the synergistic effect between R7 and PTX not only changes the penetration energy barrier but also activates the macropinocytosis and lipid raft/caveolae mediated pathway, resulting in the improvement in the translocation. The presence of heparin also improves the R7 and R7-PTX translocation. These studies provide a theoretical basis for understanding PTX delivery facilitated by the synergistic effect between CPP and cargo and paves a way for CPP design.
KeywordCell penetrating peptide Molecular dynamic simulation Synergistic effect Direct translocation Endocytosis
DOI10.1016/j.ejps.2021.105790
Language英语
WOS KeywordCELL-PENETRATING PEPTIDES ; ARGININE-RICH PEPTIDES ; DELIVERY ; INTERNALIZATION ; ENDOCYTOSIS ; PACLITAXEL ; MEMBRANES ; KINETICS ; DOCKING ; HEPARIN
Funding ProjectNational Natural Science Foundation of China[21506230] ; National Natural Science Foundation of China[21978001] ; National Natural Science Foundation of China[31770138] ; National Natural Science Foundation of China[31570120] ; Wanjiang Scholar Program from Anhui University of Technology ; Start Fund for Biochemical Engineering Research Center from Anhui University of Technology ; Scientific and Technological Project of Henan Province[202102310537] ; Schoollevel Research Project of Nanyang Normal University[2019ZX012]
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
Funding OrganizationNational Natural Science Foundation of China ; Wanjiang Scholar Program from Anhui University of Technology ; Start Fund for Biochemical Engineering Research Center from Anhui University of Technology ; Scientific and Technological Project of Henan Province ; Schoollevel Research Project of Nanyang Normal University
WOS IDWOS:000640914000002
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/48597
Collection中国科学院过程工程研究所
Corresponding AuthorXu, Xia
Affiliation1.Nanyang Normal Univ, Sch Life Sci & Agr Engn, Nanyang 473061, Henan, Peoples R China
2.Anhui Univ Technol, Biochem Engn Res Ctr, Maanshan 243032, Anhui, Peoples R China
3.Anhui Univ Technol, Dept Chem Biol, Sch Chem & Chem Engn, Maanshan 243032, Anhui, Peoples R China
4.Nanyang First Peoples Hosp, Dept Oncol, Nanyang 473002, Henan, Peoples R China
5.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wei, Yuping,Zhang, Caiying,Zhang, Man,et al. Insight of Synergistic Effect between CPP and Cargo on the Facilitation Mechanisms of R7-PTX Translocation: Experiments and Molecular Simulations[J]. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,2021,161:9.
APA Wei, Yuping.,Zhang, Caiying.,Zhang, Man.,Niu, Qionghong.,Hui, Fengli.,...&Xu, Xia.(2021).Insight of Synergistic Effect between CPP and Cargo on the Facilitation Mechanisms of R7-PTX Translocation: Experiments and Molecular Simulations.EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,161,9.
MLA Wei, Yuping,et al."Insight of Synergistic Effect between CPP and Cargo on the Facilitation Mechanisms of R7-PTX Translocation: Experiments and Molecular Simulations".EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES 161(2021):9.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wei, Yuping]'s Articles
[Zhang, Caiying]'s Articles
[Zhang, Man]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wei, Yuping]'s Articles
[Zhang, Caiying]'s Articles
[Zhang, Man]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wei, Yuping]'s Articles
[Zhang, Caiying]'s Articles
[Zhang, Man]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.