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Improvement of d-lactic acid productivity by introducing Escherichia coli acetyl-CoA synthesis pathway in engineered Saccharomyces cerevisiae
Zhong, Wei2,3; Yang, Maohua2; Hao, Xuemi2,4; Sharshar, Moustafa M.2,4; Wang, Qinhong1; Xing, Jianmin2,4
2021-05-15
Source PublicationJOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
ISSN0268-2575
Pages11
AbstractBACKGROUND d-Lactic acid (d-LA) is gaining increased attention as it can be applied in the food, pharmaceutical, cosmetic, polylactic acid and textile industries. Acid-tolerant Saccharomyces cerevisiae is often engineered to produce organic acids. For the pyruvate decarboxylases (PDCs) or alcohol dehydrogenase 1 (ADH1) disrupted strains, the productivity of d-LA significantly decreases due to the accumulation of acetaldehyde and acetic acid. To overcome the problem, Escherichia coli acetylating acetaldehyde dehydrogenase (A-ALD) enzyme genes (MHPF and EUTE) (EC 1.2.1.10) were integrated into the d-LA producing strain YIP-A15G12 strain (CGMCC2.5803). The function of these genes is to directly convert acetaldehyde into acetyl-CoA without energy consumption. This strain was integrated with Escherichia coli d-lactate dehydrogenase and Issatchenkia orientalis glycosylphosphatidylinositol-anchored protein IoGas1gene and several attenuated key pathway genes, including PDC1, PDC6, ADH1, ADH5, JEN1 (a monocarboxylate transporter), d-lactate dehydrogenase 1 (DLD1), l-lactate cytochrome c oxidoreductase (CYB2) and glycerol-3-phosphate dehydrogenases (GPD1, GPD2). RESULTS The strain expressions of the A-ALD enzymes effectively complemented the attenuated acetaldehyde dehydrogenase (ALD) acetyl-CoA synthetase (ACS) pathway and significantly improved the levels of acetyl-CoA and the productivity of d-LA. Finally, the resulting strainYIP-m-ald6 (CGMCC2.5826) produced 86.9 g L-1 d-LA with a yield of 0.77 g g(-1) glucose and a volumetric productivity of 1.77 g L-1 h(-1) at pH 3.58 under fed-batch fermentation conditions. CONCLUSION Introducing the E. coli acetyl-CoA synthesis pathway into the yeast strain can significantly enhance the growth of the strain and improve the d-LA productivity, as a benefit for d-LA production in industry. (c) 2021 Society of Chemical Industry (SCI).
Keywordd‐ lactic acid acetylating acetaldehyde dehydrogenase acetyl‐ CoA metabolic engineering Saccharomyces cerevisiae
DOI10.1002/jctb.6787
Language英语
WOS KeywordEFFICIENT PRODUCTION ; OLEAGINOUS YEAST ; D-LACTATE ; GENE ; GROWTH ; DEHYDROGENASE ; GLUCOSE ; FERMENTATION ; SYNTHETASE ; COENZYME
Funding ProjectNational Natural Science Foundation of China[21878307] ; National Natural Science Foundation of China[32001039] ; National Natural Science Foundation of China[31800030] ; National Natural Science Foundation of China[31872633] ; Industrial Biotechnology Program of Tianjin Municipal Science and Technology Commission[14ZCZDSY00066]
WOS Research AreaBiotechnology & Applied Microbiology ; Chemistry ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Environmental ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China ; Industrial Biotechnology Program of Tianjin Municipal Science and Technology Commission
WOS IDWOS:000650602400001
PublisherWILEY
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/48674
Collection中国科学院过程工程研究所
Corresponding AuthorWang, Qinhong; Xing, Jianmin
Affiliation1.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, CAS Key Lab Green Proc & Engn, Beijing, Peoples R China
3.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen Key Lab Synthet Genom,Shenzhen Inst Synt, Guangdong Prov Key Lab Synthet Genom,CAS Key Lab, Shenzhen, Peoples R China
4.Univ Chinese Acad Sci, Coll Chem Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Wei,Yang, Maohua,Hao, Xuemi,et al. Improvement of d-lactic acid productivity by introducing Escherichia coli acetyl-CoA synthesis pathway in engineered Saccharomyces cerevisiae[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2021:11.
APA Zhong, Wei,Yang, Maohua,Hao, Xuemi,Sharshar, Moustafa M.,Wang, Qinhong,&Xing, Jianmin.(2021).Improvement of d-lactic acid productivity by introducing Escherichia coli acetyl-CoA synthesis pathway in engineered Saccharomyces cerevisiae.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,11.
MLA Zhong, Wei,et al."Improvement of d-lactic acid productivity by introducing Escherichia coli acetyl-CoA synthesis pathway in engineered Saccharomyces cerevisiae".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2021):11.
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