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Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii
Zhang, Yuhan1,2; Li, Xiaolian2; Wang, Ziqiang2; Wang, Yunshan2; Ma, Yuanyuan1; Su, Zhiguo2
2021-05-15
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Pages17
AbstractThe metabolic processes involved in simultaneous production of vitamin B-12 and propionic acid by Propionibacterium freudenreichii are very complicated. To further investigate the regulatory mechanism of this metabolism, a simplified metabolic network was established. The effects of glucose feeding, propionic acid removal, and 5,6-dimethylbenzimidazole (DMB) addition on the metabolic flux distribution were investigated. The results showed that synthesis of propionic acid can be increased by increasing the metabolic flux through the oxaloacetate and methylmalonyl-CoA branches in the early and middle stages of the coupled fermentation. After DMB addition, the synthesis of vitamin B-12 was significantly enhanced via increased metabolic flux through the delta-aminolevulinate branch, which promoted the synthesis of uroporphyrinogen III, a precursor of vitamin B-12. Therefore, the analysis of metabolic flux at key nodes can provide theoretical guidance for the optimization of P. freudenreichii fermentation processes. In an experimental coupled fermentation process, the concentrations of vitamin B-12 and propionic acid reached 21.6 and 50.12 g/L respectively, increased by 105.71% and 73.91% compared with batch fermentation, which provides a new strategy for industrial production.
KeywordPropionibacterium freudenreichii Vitamin B-12 Propionic acid Membrane separation coupled fermentation Metabolic flux analysis
DOI10.1007/s12010-021-03584-y
Language英语
WOS KeywordMICROBIAL-PRODUCTION ; COBALAMIN VITAMIN-B-12 ; IMMOBILIZED CELLS ; ESCHERICHIA-COLI ; BIOSYNTHESIS ; ACIDIPROPIONICI ; GLYCEROL ; SHERMANII ; BATCH
Funding ProjectNational Natural Science Foundation of China[21506227]
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000650521200001
PublisherSPRINGER
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/48705
Collection中国科学院过程工程研究所
Corresponding AuthorWang, Ziqiang; Ma, Yuanyuan
Affiliation1.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yuhan,Li, Xiaolian,Wang, Ziqiang,et al. Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2021:17.
APA Zhang, Yuhan,Li, Xiaolian,Wang, Ziqiang,Wang, Yunshan,Ma, Yuanyuan,&Su, Zhiguo.(2021).Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,17.
MLA Zhang, Yuhan,et al."Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2021):17.
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