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Improved stability of the carbon nanotubes-enzyme bioconjugates by biomimetic silicification
Wang, Qi1,2; Zhou, Liya1; Jiang, Yanjun1,2; Gao, Jing1; Gao, J
2011-06-10
Source PublicationENZYME AND MICROBIAL TECHNOLOGY
ISSN0141-0229
Volume49Issue:1Pages:11-16
AbstractNano-materials have been applied in many fields due to their excellent characteristics, such as the high surface area-to-volume ratio, excellent physicochemical properties and biological compatibility. In this study, multi-walled carbon nanotubes (MWCNTs) were utilized to prepare MWCNTs-papain bioconjugates and then realized the immobilization of papain. MWCNTs functionalized with carboxyl- and amine- groups on their surface were used as immobilization carriers. The immobilization of papain on the functionalized MWCNTs through physical absorption was examined. The conjugates were denoted as MWCNTs-papain bioconjugates. To improve the stability, the bioconjugates were further coated by silica through the biomimetic silicification process that induced by papain (denoted as silica-coated bioconjugates). The as-prepared MWCNTs-papain bioconjugates and the silica-coated bioconjugates were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The preliminary results showed that the bioconjugates could retain most of the initial activity of papain. Compared to free papain and MWCNTs-papain bioconjugates, the silica-coated bioconjugates exhibited significantly improved thermal, pH and recycling stability. Comparisons of the kinetic parameters between MWCNTs-papain bioconjugates and the silica-coated bioconjugates revealed that the K(m), value of the immobilized papain experienced a slight increase after silica coating, which suggested that the silica coating did not significantly hinder papain's access to substrate or release of product. (C) 2011 Elsevier Inc. All rights reserved.
KeywordBioconjugate Multi-walled Carbon Nanotubes Biomimetic Silicification Papain Immobilization
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.enzmictec.2011.04.007
Indexed BySCI
Language英语
WOS KeywordPAPAIN ; IMMOBILIZATION ; CHITOSAN ; SILICA ; COMPOSITES
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000292231500003
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4877
Collection生化工程国家重点实验室
Corresponding AuthorGao, J
Affiliation1.Hebei Univ Technol, Sch Chem Engn, Tianjin 300130, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Qi,Zhou, Liya,Jiang, Yanjun,et al. Improved stability of the carbon nanotubes-enzyme bioconjugates by biomimetic silicification[J]. ENZYME AND MICROBIAL TECHNOLOGY,2011,49(1):11-16.
APA Wang, Qi,Zhou, Liya,Jiang, Yanjun,Gao, Jing,&Gao, J.(2011).Improved stability of the carbon nanotubes-enzyme bioconjugates by biomimetic silicification.ENZYME AND MICROBIAL TECHNOLOGY,49(1),11-16.
MLA Wang, Qi,et al."Improved stability of the carbon nanotubes-enzyme bioconjugates by biomimetic silicification".ENZYME AND MICROBIAL TECHNOLOGY 49.1(2011):11-16.
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