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Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide
Rozhina, Elvira1; Batasheva, Svetlana1; Miftakhova, Regina1; Yan, Xuehai2; Vikulina, Anna3; Volodkin, Dmitry4; Fakhrullin, Rawil1
2021-05-01
Source PublicationAPPLIED CLAY SCIENCE
ISSN0169-1317
Volume205Pages:10
AbstractThis study aimed at comparative examining of the interactions between conventionally used clay and carbon nanomaterials and human lung adenocarcinoma cells (A549 cells). The following platy and tubular nanomaterials were tested: carbon nanoparticles, i.e. multi-walled carbon nanotubes (MWCNTs) and graphene oxide nanosheets (GO) as well as nanoclays, i.e. halloysite nanotubes (HNTs) and kaolinite nanosheets (Kaol). Nanoparticle physicochemical properties and their internalization into cells were examined using dynamic light scattering as well as atomic force, 3D laser scanning confocal and darkfield hyperspectral microscopies. Biological aspects of the nanomaterial-cell interaction included assessment of cellular toxicity, DNA damage, metabolic activity, and physical parameters of the cells. Regardless of a shape, carbon nanomaterials demonstrated cell surface adsorption, but negligible penetration into cells compared to nanoclays. However, carbon nanomaterials were found to be the most toxic for cells as probed by the MTS assay. They also turned out to be the most genotoxic for cells compared to nanoclays as revealed by the DNA-Comet assay. GO significantly increased the fraction of apoptotic cells and was the most cytotoxic and genotoxic nanomaterial. Comparison of flow cytometry and MTS data indicated that a cytotoxic effect of MWCNTs was not associated with increased cell death, but was rather due to a decrease in cell metabolic activity and/or proliferation. Finally, no significant effect of the shape of the tested nanomaterials on their internalization and cytotoxicity was revealed.
KeywordHalloysite Kaolinite Multiwalled carbon nanotubes Graphene oxide Cytotoxicity Genotoxicity
DOI10.1016/j.clay.2021.106041
Language英语
WOS KeywordDNA-DAMAGE ; IN-VITRO ; OXIDATIVE STRESS ; CELLULAR TOXICITY ; COMET ASSAY ; NANOPARTICLES ; GENOTOXICITY ; DELIVERY ; SURFACE ; ADSORPTION
Funding ProjectRFBR[18-53-80067 BRICS_t] ; National Natural Science Fund BRICS STI Framework Program of China[51861145304]
WOS Research AreaChemistry ; Materials Science ; Mineralogy
WOS SubjectChemistry, Physical ; Materials Science, Multidisciplinary ; Mineralogy
Funding OrganizationRFBR ; National Natural Science Fund BRICS STI Framework Program of China
WOS IDWOS:000647564700006
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/48779
Collection中国科学院过程工程研究所
Corresponding AuthorFakhrullin, Rawil
Affiliation1.Kazan Fed Univ, Inst Fundamental Med & Biol, Kreml Uram 18, Kazan 420008, Republic Of Tat, Russia
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Fraunhofer Inst Cell Therapy & Immunol, Branch Bioanalyt & Bioproc, Muhlenberg 13, D-14476 Potsdam, Germany
4.Nottingham Trent Univ, Sch Sci & Technol, Dept Chem & Forens, Clifton Lane, Nottingham NG11 8NS, England
Recommended Citation
GB/T 7714
Rozhina, Elvira,Batasheva, Svetlana,Miftakhova, Regina,et al. Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide[J]. APPLIED CLAY SCIENCE,2021,205:10.
APA Rozhina, Elvira.,Batasheva, Svetlana.,Miftakhova, Regina.,Yan, Xuehai.,Vikulina, Anna.,...&Fakhrullin, Rawil.(2021).Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide.APPLIED CLAY SCIENCE,205,10.
MLA Rozhina, Elvira,et al."Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide".APPLIED CLAY SCIENCE 205(2021):10.
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