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A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography
Qu, Jian-Bo1; Huang, Yong-Dong2; Jing, Guang-Lun1; Liu, Jian-Guo1; Zhou, Wei-Qing2; Zhu, Hu1; Lu, Jian-Ren1; Qu, JB
2011-05-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume879Issue:15-16Pages:1043-1048
AbstractAgarose coated gigaporous polystyrene microspheres were evaluated as a novel matrix for immobilized-metal affinity chromatography (IMAC). With four steps, nickel ions were successfully immobilized on the microspheres. The gigaporous structure and chromatographic properties of IMAC medium were characterized. A column packed with the matrix showed low column backpressure and high column efficiency at high flow velocity. Furthermore, this matrix was used for purifying superoxide dismutase (SOD), which was expressed in Escherichia coli (E. coli) in submerged fermentation, on an Akta purifier 100 system under different flow velocities. The purity of the SOD from this one-step purification was 79% and the recovery yield was about 89.6% under the superficial flow velocity of 3251 cm/h. In conclusion, all the results suggested that the gigaporous matrix has considerable advantages for high-speed immobilized-metal affinity chromatography. (C) 2011 Elsevier B.V. All rights reserved.
KeywordGigaporous Polystyrene Microspheres Column Efficiency Immobilized-metal Affinity Chromatography Superoxide Dismutase
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2011.03.015
Indexed BySCI
Language英语
WOS KeywordPERFUSION CHROMATOGRAPHY ; PROTEIN CHROMATOGRAPHY ; PURIFICATION ; SEPARATION ; DISMUTASE ; MEDIA
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000290645400003
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4882
Collection生化工程国家重点实验室
Corresponding AuthorQu, JB
Affiliation1.China Univ Petr E China, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Qu, Jian-Bo,Huang, Yong-Dong,Jing, Guang-Lun,et al. A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(15-16):1043-1048.
APA Qu, Jian-Bo.,Huang, Yong-Dong.,Jing, Guang-Lun.,Liu, Jian-Guo.,Zhou, Wei-Qing.,...&Qu, JB.(2011).A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(15-16),1043-1048.
MLA Qu, Jian-Bo,et al."A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.15-16(2011):1043-1048.
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