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One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel
Liu, Dan2; Ma, Yan3; Wang, Ye4; Su, Zhiguo1; Gu, Ming1; Janson, Jan-Christer5; Gu, M
2011-05-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume34Issue:9Pages:995-998
AbstractThe hydrolysable tannins corilagin and geraniin, the major active components of the traditional Chinese medicine Geranium wilfordii Maxim, have been separated and purified from crude extracts in one step by adsorption chromatography on cross-linked 12% agarose gel (Superose 12 10/300 GL). The separation was achieved by gradient elution using mobile phase A composed of 5% ethanol and 5% acetic acid and mobile phase B composed of 30% ethanol and 30% acetic acid. The gradients were composed as follows: 0-240 mL, 0-25% B; 240-480 mL, 25-40% B; after 480 mL, 100% B. The purities of the collected corilagin and geraniin were 92.4 and 87.2%, and the corresponding yields were 88.0 and 76.8%, respectively.
KeywordAdsorption Chromatography Cross-linked Agarose Gel Hydrolysable Tannin
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/jssc.201100006
Indexed BySCI
Language英语
WOS KeywordCENTRIFUGAL PARTITION CHROMATOGRAPHY ; COUNTER-CURRENT CHROMATOGRAPHY ; CORILAGIN ; MEDIA ; ACID ; L. ; POLYPHENOLS ; ANTIOXIDANT ; CHEMISTRY ; GALLATE
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000290634600004
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4883
Collection生化工程国家重点实验室
Corresponding AuthorGu, M
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian, Peoples R China
3.Jiangsu Membrane Separat Co Ltd, Dalian, Peoples R China
4.Nanjing Agr Univ, Nanjing, Peoples R China
5.Uppsala Univ, Uppsala, Sweden
Recommended Citation
GB/T 7714
Liu, Dan,Ma, Yan,Wang, Ye,et al. One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(9):995-998.
APA Liu, Dan.,Ma, Yan.,Wang, Ye.,Su, Zhiguo.,Gu, Ming.,...&Gu, M.(2011).One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel.JOURNAL OF SEPARATION SCIENCE,34(9),995-998.
MLA Liu, Dan,et al."One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel".JOURNAL OF SEPARATION SCIENCE 34.9(2011):995-998.
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