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Application of reversed-phase liquid chromatography in separation and analysis of proteins and peptides
Alternative TitleChin. J. Anal. Chem.
Zhu, XN; Su, ZG
2004-02-01
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume32Issue:2Pages:248-254
AbstractReversed-phase liquid chromatography (RPLC) is based on the hydrophobic interaction, and has found increasing use in analysis and separation of bio-molecules due to its high resolution, reproducibility and recovery. In this review, the mechanism of RPLC is discussed and the applications of RPLC in the study of proteins and peptides are summarized. Adsorption and desorption of proteins by RPLC are described with an "on-off" mechanism, which states the interactions between the proteins, the molecules in the mobile phase and the stationary phase. The applications of RPLC are advanced in recent years, including protein and peptide purification, peptide mapping analysis, enzyme activity assay, conformational change detection and hydrophobic interaction study. The prospect of reversed-phase liquid chromatography in this field is anticipated.; Reversed-phase liquid chromatography (RPLC) is based on the hydrophobic interaction, and has found increasing use in analysis and separation of bio-molecules due to its high resolution, reproducibility and recovery. In this review, the mechanism of RPLC is discussed and the applications of RPLC in the study of proteins and peptides are summarized. Adsorption and desorption of proteins by RPLC are described with an "on-off" mechanism, which states the interactions between the proteins, the molecules in the mobile phase and the stationary phase. The applications of RPLC are advanced in recent years, including protein and peptide purification, peptide mapping analysis, enzyme activity assay, conformational change detection and hydrophobic interaction study. The prospect of reversed-phase liquid chromatography in this field is anticipated.
KeywordReversed-phase Liquid Chromatography Protein Peptide Purification Analysis Review
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
URL查看原文
Indexed BySCI
Language英语
WOS KeywordMASS-SPECTROMETRY ; STATIONARY PHASES ; ESCHERICHIA-COLI ; RETENTION ; PURIFICATION ; IDENTIFICATION ; BEHAVIOR ; SEQUENCE ; PROTEOME ; ASSAY
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000221026100030
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4896
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhu, XN,Su, ZG. Application of reversed-phase liquid chromatography in separation and analysis of proteins and peptides[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2004,32(2):248-254.
APA Zhu, XN,&Su, ZG.(2004).Application of reversed-phase liquid chromatography in separation and analysis of proteins and peptides.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,32(2),248-254.
MLA Zhu, XN,et al."Application of reversed-phase liquid chromatography in separation and analysis of proteins and peptides".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 32.2(2004):248-254.
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