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| Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship | |
| Fan, Qingze1,2; Miao, Chunyu2; Huang, Yilan1; Yue, Hua2; Wu, Anguo3; Wu, Jianming3; Wu, Jie2; Ma, Guanghui2 | |
| 2021-08-15 | |
| Source Publication | CARBOHYDRATE POLYMERS
 |
| ISSN | 0144-8617 |
| Volume | 266Pages:10 |
| Abstract | In this study, 2-hydroxypropyltrimethyl ammonium chloride chitosan (HTCC)-based hydrogel was devised as a mucosal adjuvant for H5N1 vaccine. Aimed to investigate the structure activity relationship between HTCC hydrogel and immune response, we prepared a series of HTCC hydrogel with defined quaternization degrees (DQs, 0%, 21%, 41%, 60%, 80%). Results suggested that with DQ increasing, the positive charge and gelation time of HTCC hydrogel increased but the viscosity decreased. We applied in vivo imaging system and found that the moderate DQ 41% prolonged antigen residence time in nasal cavity, resulting in the most potent systemic responses (IgG, IgG1, IgG2a, HI). While, the lowest DQ 0% produced the best mucosal IgA antibody responses, most likely due to the closer contact with mucosa. Furthermore, the influence of animal gender was also discussed. These data add to the growing understanding of the relationship between physicochemical features of chitosan-based hydrogel and how they influence the immune responses. |
| Keyword | Chitosan-based hydrogel Structure activity relationship Intranasal immunization Adjuvant |
| DOI | 10.1016/j.carbpol.2021.118139 |
| Language | 英语 |
| WOS Keyword | N-TRIMETHYL CHITOSAN ; IMMUNE-RESPONSE ; CLEARANCE CHARACTERISTICS ; QUATERNIZED CHITOSAN ; VACCINE ; VIRUS ; MICE ; TMC ; IMMUNOGENICITY ; TRANSMISSION |
| Funding Project | National Key Research & Development Program of China[2018YFC0311303] ; National Natural Science Foundation of China (NSFC)[21776287] ; National Natural Science Foundation of China (NSFC)[22078335] ; Luzhou Science and Technology Program[2018-JYJ-41] ; Foundation of Southwest Medical University[2017-ZRZD-003] ; Southwest Medical University[2017-LH011] ; Southwest Medical University[2018LZXNYD-PT02] ; Ph.D. Research Startup Foundation of the Affiliated Hospital of Southwest Medical University[17136] ; Collaborative Project of Luzhou TCM Hospital[2017-LH011] ; Collaborative Project of Luzhou Government[2018LZXNYD-PT02] |
| WOS Research Area | Chemistry ; Polymer Science |
| WOS Subject | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| Funding Organization | National Key Research & Development Program of China ; National Natural Science Foundation of China (NSFC) ; Luzhou Science and Technology Program ; Foundation of Southwest Medical University ; Southwest Medical University ; Ph.D. Research Startup Foundation of the Affiliated Hospital of Southwest Medical University ; Collaborative Project of Luzhou TCM Hospital ; Collaborative Project of Luzhou Government |
| WOS ID | WOS:000655700400006 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/49034 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Wu, Jie; Ma, Guanghui |
| Affiliation | 1.Southwest Med Univ, Dept Pharm, Affiliated Hosp, Luzhou 646000, Sichuan, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 Bei Er Jie, Beijing 100190, Peoples R China 3.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Luzhou 646000, Sichuan, Peoples R China |
| Recommended Citation GB/T 7714 | Fan, Qingze,Miao, Chunyu,Huang, Yilan,et al. Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship[J]. CARBOHYDRATE POLYMERS,2021,266:10. |
| APA | Fan, Qingze.,Miao, Chunyu.,Huang, Yilan.,Yue, Hua.,Wu, Anguo.,...&Ma, Guanghui.(2021).Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship.CARBOHYDRATE POLYMERS,266,10. |
| MLA | Fan, Qingze,et al."Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship".CARBOHYDRATE POLYMERS 266(2021):10. |
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