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Cell culture of Saussurea medusa in a periodically submerged air-lift bioreactor
Alternative TitleBiochem. Eng. J.
Yuan, XF; Zhao, B; Wang, YC
2004-11-01
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume21Issue:3Pages:235-239
AbstractCell culture of Saussurea medusa in a 2-1 periodically submerged air-lift bioreactor (PSAB) was investigated. The periodically unsubmerged (immobilization culture) and submerged (suspension culture) operation of the whole bioreactor system was controlled by the electromagnetic valves and driven rod controller connected with the time controller. The maximum cell biomass (10.4 gl(-1)), content of total flavonoids (4.8%), production of total flavonoids (501 mgl(-1)) and the optimal size distribution of cell aggregates were obtained under the optimal unsubmerged/submerged period of 10 min/8 h (the cell inoculation size was 1.0 gl(-1), and the air flowrate was 20/401 h(-1)). Though the content of total flavonoids was still a little lower than that obtained from the shake flask culture, the specific growth rate of cells in PSAB was 2.1-fold and the production of total flavonoids was 1.7-fold of those in shake flasks. It was concluded that PSAB had advantages in improving cell growth of S. medusa and the production of total flavonoids. (C) 2004 Elsevier B.V. All rights reserved.; Cell culture of Saussurea medusa in a 2-1 periodically submerged air-lift bioreactor (PSAB) was investigated. The periodically unsubmerged (immobilization culture) and submerged (suspension culture) operation of the whole bioreactor system was controlled by the electromagnetic valves and driven rod controller connected with the time controller. The maximum cell biomass (10.4 gl(-1)), content of total flavonoids (4.8%), production of total flavonoids (501 mgl(-1)) and the optimal size distribution of cell aggregates were obtained under the optimal unsubmerged/submerged period of 10 min/8 h (the cell inoculation size was 1.0 gl(-1), and the air flowrate was 20/401 h(-1)). Though the content of total flavonoids was still a little lower than that obtained from the shake flask culture, the specific growth rate of cells in PSAB was 2.1-fold and the production of total flavonoids was 1.7-fold of those in shake flasks. It was concluded that PSAB had advantages in improving cell growth of S. medusa and the production of total flavonoids. (C) 2004 Elsevier B.V. All rights reserved.
KeywordBioreactor Engineering Flavonoids Periodically Submerged Airlift Bioreactor (Psab) Plant Cell Culture Saussurea Medusa Secondary Metabolite
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2004.07.003
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000224295400006
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4905
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yuan, XF,Zhao, B,Wang, YC. Cell culture of Saussurea medusa in a periodically submerged air-lift bioreactor[J]. BIOCHEMICAL ENGINEERING JOURNAL,2004,21(3):235-239.
APA Yuan, XF,Zhao, B,&Wang, YC.(2004).Cell culture of Saussurea medusa in a periodically submerged air-lift bioreactor.BIOCHEMICAL ENGINEERING JOURNAL,21(3),235-239.
MLA Yuan, XF,et al."Cell culture of Saussurea medusa in a periodically submerged air-lift bioreactor".BIOCHEMICAL ENGINEERING JOURNAL 21.3(2004):235-239.
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