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| Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine | |
| Luo, Ruoshi1,2; Qin, Zhao2; Zhou, Dan2; Wang, Dan1,2; Hu, Ge2; Su, Zhiguo3; Zhang, Suojiang3 | |
| 2021-06-10 | |
| Source Publication | ENGINEERING IN LIFE SCIENCES
 |
| ISSN | 1618-0240 |
| Pages | 7 |
| Abstract | Nylon is a polyamide material with excellent performance used widely in the aviation and automobile industries, and other fields. Nylon monomers such as hexamethylene diamine and other monomers are in huge demand. Therefore, in order to expand the methods of nylon production, we tried to develop alternative bio-manufacturing processes which would make a positive contribution to the nylon industry. In this study, the engineered E. coli-overexpressing Lysine decarboxylases (LDCs) were used for the bioconversion of l-lysine to cadaverine. An integrated fermentation and microfiltration (MF) process for high-level cadaverine production by E. coli was established. Concentration was increased from 87 to 263.6 g/L cadaverine after six batch coupling with a productivity of 3.65 g/L-h. The cadaverine concentration was also increased significantly from 0.43 g cadaverine/g l-lysine to 0.88 g cadaverine/g l-lysine by repeated batch fermentation. These experimental results indicate that coupling the fermentation and membrane separation process could benefit the continuous production of cadaverine at high levels. |
| Keyword | batch fermentation cadaverine lysine decarboxylases membrane separation polyamides monomer |
| DOI | 10.1002/elsc.202000099 |
| Language | 英语 |
| WOS Keyword | LACTIC-ACID PRODUCTION ; ESCHERICHIA-COLI ; SUCCINIC ACID |
| Funding Project | National Natural Science Foundation of China[21978027] ; Fundamental Research Funds for the Central Universities[2020CDCGJ020] ; Fundamental Research Funds for the Central Universities[2020CDCGHG068] ; Scientific Research Foundation of State Key Lab. of Coal Mine Disaster Dynamics and Control[2011DA105287-ZR202002] ; Key Projects of Technological Innovation and Application Development of Chongqing, China[cstc 2019jscx-fxydX0024] |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| Funding Organization | National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Scientific Research Foundation of State Key Lab. of Coal Mine Disaster Dynamics and Control ; Key Projects of Technological Innovation and Application Development of Chongqing, China |
| WOS ID | WOS:000659724800001 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/49073 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Wang, Dan |
| Affiliation | 1.Chongqing Univ, State Key Lab Coal Mine Disaster Dynam & Control, Chongqing 401331, Peoples R China 2.Chongqing Univ, Dept Chem Engn, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Luo, Ruoshi,Qin, Zhao,Zhou, Dan,et al. Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine[J]. ENGINEERING IN LIFE SCIENCES,2021:7. |
| APA | Luo, Ruoshi.,Qin, Zhao.,Zhou, Dan.,Wang, Dan.,Hu, Ge.,...&Zhang, Suojiang.(2021).Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine.ENGINEERING IN LIFE SCIENCES,7. |
| MLA | Luo, Ruoshi,et al."Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine".ENGINEERING IN LIFE SCIENCES (2021):7. |
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