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CMCase activity assay as a method for cellulase adsorption analysis
Alternative TitleEnzyme Microb. Technol.
Zhou, XH; Chen, HZ; Li, ZH
2004-10-06
Source PublicationENZYME AND MICROBIAL TECHNOLOGY
ISSN0141-0229
Volume35Issue:5Pages:455-459
AbstractMicrocrystalline cellulose (MCC) was hydrolyzed with Trichoderma viride cellulase, linear correlation was found between free filter paper activity (FPA) and free carboxymethyl cellulase activity (CA), and between bound CA and bound protein concentration at 0-72 h reaction time. Linear correlation between bound FPA and bound protein concentration was found at short reaction time (I h), but not found at long reaction time. CA, the activity of endoglucanase, may be used to describe the adsorption of whole cellulase, at least, for MCC-T viride cellulase system. and the error for CA assay was less than which for FPA assay and protein concentration assay in hydrolysis condition. (C) 2004 Elsevier Inc. All rights reserved.; Microcrystalline cellulose (MCC) was hydrolyzed with Trichoderma viride cellulase, linear correlation was found between free filter paper activity (FPA) and free carboxymethyl cellulase activity (CA), and between bound CA and bound protein concentration at 0-72 h reaction time. Linear correlation between bound FPA and bound protein concentration was found at short reaction time (I h), but not found at long reaction time. CA, the activity of endoglucanase, may be used to describe the adsorption of whole cellulase, at least, for MCC-T viride cellulase system. and the error for CA assay was less than which for FPA assay and protein concentration assay in hydrolysis condition. (C) 2004 Elsevier Inc. All rights reserved.
KeywordCmcase Activity Assay Filter Paper Activity Assay Protein Concentration Assay Cellulase Adsorption
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.enzmictec.2004.07.005
URL查看原文
Indexed BySCI
Language英语
WOS KeywordTRICHODERMA-REESEI ; HYDROLYSIS ; COMPONENTS ; SYNERGISM ; ACID
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000224142200013
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4908
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhou, XH,Chen, HZ,Li, ZH. CMCase activity assay as a method for cellulase adsorption analysis[J]. ENZYME AND MICROBIAL TECHNOLOGY,2004,35(5):455-459.
APA Zhou, XH,Chen, HZ,&Li, ZH.(2004).CMCase activity assay as a method for cellulase adsorption analysis.ENZYME AND MICROBIAL TECHNOLOGY,35(5),455-459.
MLA Zhou, XH,et al."CMCase activity assay as a method for cellulase adsorption analysis".ENZYME AND MICROBIAL TECHNOLOGY 35.5(2004):455-459.
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