CAS OpenIR
Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens
Hu, Qiushi1,2; Wu, Qixiao2; Huang, Fengchun1; Xu, Zheng2; Zhou, Lei1; Zhao, Suling2
2021-06-16
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume13Issue:23Pages:26782-26789
AbstractTechnologies for rapid screening of multiple foodborne pathogens have been urgently needed because of the complex food matrix and high outbreaks of foodborne diseases. In this study, multicolor coding up-conversion nanoparticles (UCNPs) were synthesized and applied for rapid and simultaneous detection of five kinds of foodborne pathogens. The multicolor coding UCNPs were obtained through doping different concentrations of a sensitizer (Yb3+) on the shell of the synthesized NaYF4:Yb3+, Tm3+ (20%/2%)@NaYF4:Yb3+, and Er3+ (x %/2%) core/shell nanocrystals. All the UCNPs could emit red and green luminescence simultaneously once excited with near-infrared wavelength (980 nm), and the ratio of red and green (R/G ratio) emission light intensity of each kind of UCNPs varied depending on the Yb3+ doping concentration. In addition, the magnetic nanoparticles (MNPs) modified with the monoclonal antibodies (mAbs) against the target bacteria were used to capture and separate the bacteria, resulting in obtaining the MNP-bacterium complexes. Different UCNPs with multicolor coding acted as signal probes were also modified with the mAbs to react with the MNP-bacterium complexes to form the MNP-bacterium-UCNP sandwich complexes. After the sandwich complexes were excited with a wavelength of 980 nm, the obtained R/G ratios and the green photoluminescence intensity (PL intensity) could be used to distinguish and quantitatively detect foodborne pathogens, respectively. This proposed nanoplatform could detect five foodborne pathogens simultaneously within 2 h with good sensitivity and specificity, showing great potential for multiplex detection of other targets in the fields of medical diagnosis and food security.
Keywordmulticolor coding up-conversion nanoparticles photoluminescence multiplex detection foodborne pathogen
DOI10.1021/acsami.1c05522
Language英语
WOS KeywordQUANTITATIVE DETECTION ; SALMONELLA ; NANOCRYSTALS ; BACTERIA ; ASSAY ; PCR ; RED ; LUMINESCENCE ; MICROARRAYS ; EMISSION
Funding ProjectNational Key Research and Development Program[2019YFC1606600] ; National Key Research and Development Program[2019YFC1606602] ; Beijing Nova Programme Interdisciplinary Cooperation Project[Z191100001119005] ; Independent Research Project of State Key Laboratory of Biochemical Engineering - Innovation Academy for Green Manufacture, Chinese Academy of Sciences[2020ZZ-03] ; Independent Research Project of State Key Laboratory of Biochemical Engineering - Innovation Academy for Green Manufacture, Chinese Academy of Sciences[IAGM2020C31] ; [BWS18C015] ; [18-163-12-ZT-005-016-01] ; [19-H863-18-ZD-011-004-03] ; [17SAZ08] ; [2019-JCJQ-ZD-117-05-1] ; [20-163-12-ZT-006-003-02]
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
Funding OrganizationNational Key Research and Development Program ; Beijing Nova Programme Interdisciplinary Cooperation Project ; Independent Research Project of State Key Laboratory of Biochemical Engineering - Innovation Academy for Green Manufacture, Chinese Academy of Sciences
WOS IDWOS:000664289800012
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/49289
Collection中国科学院过程工程研究所
Corresponding AuthorZhou, Lei; Zhao, Suling
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Beijing Jiaotong Univ, Inst Optoelect Technol, Key Lab Luminescence & Opt Informat, Minist Educ, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Hu, Qiushi,Wu, Qixiao,Huang, Fengchun,et al. Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens[J]. ACS APPLIED MATERIALS & INTERFACES,2021,13(23):26782-26789.
APA Hu, Qiushi,Wu, Qixiao,Huang, Fengchun,Xu, Zheng,Zhou, Lei,&Zhao, Suling.(2021).Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens.ACS APPLIED MATERIALS & INTERFACES,13(23),26782-26789.
MLA Hu, Qiushi,et al."Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens".ACS APPLIED MATERIALS & INTERFACES 13.23(2021):26782-26789.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hu, Qiushi]'s Articles
[Wu, Qixiao]'s Articles
[Huang, Fengchun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hu, Qiushi]'s Articles
[Wu, Qixiao]'s Articles
[Huang, Fengchun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hu, Qiushi]'s Articles
[Wu, Qixiao]'s Articles
[Huang, Fengchun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.