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Fingerprinting of Salvia miltiorrhiza Bunge by non-aqueous capillary electrophoresis compared with high-speed counter-current chromatography
Alternative TitleJ. Chromatogr. A
Gu, M; Zhang, SF; Su, ZG; Chen, Y; Fan, OY
2004-11-19
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1057Issue:1-2Pages:133-140
AbstractThe component of the traditional Chinese medicine (TCM) can be influenced by soil, climate, and growth stage, and fingerprint is an important means in its quality control. Our previous studies showed that high-speed counter-current chromatography (HSCCC) was helpful in the development of fingerprint of TCM. Since the HSCCC method is new, it is necessary to compare it with conventional ones, such as high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC) and high-performance capillary electrophoresis (HPCE). Comparison with HPLC was conducted in our previous study. In this study, HSCCC was compared with non-aqueous capillary electrophoresis (NACE). With NACE, seven stable components were separated within 55 min, respectively, from three crude samples of Salvia miltiorrhiza Bunge from different growth locations. In HSCCC separation, 12 components were separated, respectively, with good correspondence and precision within 13 h. Both NACE and HSCCC were effective in showing whole concentration distribution of all kinds of constituents. Principles of these two methods were very different, which led to different elution sequences and relative contents of peaks. HSCCC showed better performance in analysis of tanshinones, which made its fingerprint containing more chemical information than that of NACE. It was further proven that HSCCC could be a feasible and cost-effective method in the development of the fingerprint of TCM. (C) 2004 Elsevier B.V. All rights reserved.; The component of the traditional Chinese medicine (TCM) can be influenced by soil, climate, and growth stage, and fingerprint is an important means in its quality control. Our previous studies showed that high-speed counter-current chromatography (HSCCC) was helpful in the development of fingerprint of TCM. Since the HSCCC method is new, it is necessary to compare it with conventional ones, such as high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC) and high-performance capillary electrophoresis (HPCE). Comparison with HPLC was conducted in our previous study. In this study, HSCCC was compared with non-aqueous capillary electrophoresis (NACE). With NACE, seven stable components were separated within 55 min, respectively, from three crude samples of Salvia miltiorrhiza Bunge from different growth locations. In HSCCC separation, 12 components were separated, respectively, with good correspondence and precision within 13 h. Both NACE and HSCCC were effective in showing whole concentration distribution of all kinds of constituents. Principles of these two methods were very different, which led to different elution sequences and relative contents of peaks. HSCCC showed better performance in analysis of tanshinones, which made its fingerprint containing more chemical information than that of NACE. It was further proven that HSCCC could be a feasible and cost-effective method in the development of the fingerprint of TCM. (C) 2004 Elsevier B.V. All rights reserved.
KeywordNon-aqueous Capillary Electrophoresis (Nace) High-speed Counter-current Chromatography (Hsccc) Fingerprint Salvia Miltiorrhiza Bunge Tanshinones
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2004.08.145
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; TANSHINONES ; SEPARATION ; PURIFICATION ; DITERPENOIDS ; ACID
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000225143900016
Citation statistics
Cited Times:80[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4936
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Gu, M,Zhang, SF,Su, ZG,et al. Fingerprinting of Salvia miltiorrhiza Bunge by non-aqueous capillary electrophoresis compared with high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1057(1-2):133-140.
APA Gu, M,Zhang, SF,Su, ZG,Chen, Y,&Fan, OY.(2004).Fingerprinting of Salvia miltiorrhiza Bunge by non-aqueous capillary electrophoresis compared with high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1057(1-2),133-140.
MLA Gu, M,et al."Fingerprinting of Salvia miltiorrhiza Bunge by non-aqueous capillary electrophoresis compared with high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1057.1-2(2004):133-140.
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