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Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination
Alternative TitleIn Vitro Cell. Dev. Biol.-Plant
Liu, CZ; Murch, SJ; Jain, JC; Saxena, PK
2004
Source PublicationIN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN1054-5476
Volume40Issue:1Pages:75-79
AbstractHydrastis canadensis L. (Goldenseal) is an endangered medicinal plant used in the treatment of many ailments, such as gastrointestinal disturbances, urinary disorders, hemorrhage, skin, mouth and eye infections, and inflammation. Commercial preparations of wild-harvested goldenseal were found to contain heavy metal contaminants including aluminum (848 mug g(-1)), cadmium (0.4 mug g(-1)), lead (18.7 mug g(-1)), and mercury (0.1 mug g(-1)). As well, goldenseal is an endangered species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II. Therefore, the practice of wild-harvest is actually decimating natural populations of goldenseal and endangering its genetic diversity. In vitro propagation protocol by tissue culture was developed for producing high-quality tissues of goldenseal. Significantly more de novo, regeneration was induced on stem explants of 3-mo.-old plants cultured on a medium containing 10 muM 6-benzylaminopurine (BA) (22 regenerants per explant) than any other treatment. Subculture of the regenerants on a medium devoid of growth regulators resulted in the development of, complete plants that were acclimatized and thrived in standard greenhouse conditions. The plants regenerated in vitro contained the lowest levels of heavy metals. The findings of this study provide the first evidence that heavy metal contaminants bioaccumulate in goldenseal tissues and also provide a method for germplasm conservation, mass multiplication, and production of goldenseal tissues free from abiotic contamination.; Hydrastis canadensis L. (Goldenseal) is an endangered medicinal plant used in the treatment of many ailments, such as gastrointestinal disturbances, urinary disorders, hemorrhage, skin, mouth and eye infections, and inflammation. Commercial preparations of wild-harvested goldenseal were found to contain heavy metal contaminants including aluminum (848 mug g(-1)), cadmium (0.4 mug g(-1)), lead (18.7 mug g(-1)), and mercury (0.1 mug g(-1)). As well, goldenseal is an endangered species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II. Therefore, the practice of wild-harvest is actually decimating natural populations of goldenseal and endangering its genetic diversity. In vitro propagation protocol by tissue culture was developed for producing high-quality tissues of goldenseal. Significantly more de novo, regeneration was induced on stem explants of 3-mo.-old plants cultured on a medium containing 10 muM 6-benzylaminopurine (BA) (22 regenerants per explant) than any other treatment. Subculture of the regenerants on a medium devoid of growth regulators resulted in the development of, complete plants that were acclimatized and thrived in standard greenhouse conditions. The plants regenerated in vitro contained the lowest levels of heavy metals. The findings of this study provide the first evidence that heavy metal contaminants bioaccumulate in goldenseal tissues and also provide a method for germplasm conservation, mass multiplication, and production of goldenseal tissues free from abiotic contamination.
KeywordHydrastis Canadensis l Medicinal Plant Plant Regeneration Heavy Metal Contamination
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1079/IVP2003499
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCULTURES
WOS Research AreaPlant Sciences ; Cell Biology ; Developmental Biology
WOS SubjectPlant Sciences ; Cell Biology ; Developmental Biology
WOS IDWOS:000220431300009
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4938
Collection研究所(批量导入)
Affiliation1.Univ Guelph, Dept Plant Agr, Div Biotechnol, Guelph, ON N1G 2W1, Canada
2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
3.Natl Trop Bot Garden, Inst Ethnobot, Kalaheo, HI USA
4.Univ Notre Dame, Dept Civil Engn & Geol Sci, Notre Dame, IN 46556 USA
Recommended Citation
GB/T 7714
Liu, CZ,Murch, SJ,Jain, JC,et al. Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2004,40(1):75-79.
APA Liu, CZ,Murch, SJ,Jain, JC,&Saxena, PK.(2004).Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,40(1),75-79.
MLA Liu, CZ,et al."Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 40.1(2004):75-79.
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