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| Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination | |
| Alternative Title | In Vitro Cell. Dev. Biol.-Plant |
| Liu, CZ; Murch, SJ; Jain, JC; Saxena, PK | |
| 2004 | |
| Source Publication | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| ISSN | 1054-5476 |
| Volume | 40Issue:1Pages:75-79 |
| Abstract | Hydrastis canadensis L. (Goldenseal) is an endangered medicinal plant used in the treatment of many ailments, such as gastrointestinal disturbances, urinary disorders, hemorrhage, skin, mouth and eye infections, and inflammation. Commercial preparations of wild-harvested goldenseal were found to contain heavy metal contaminants including aluminum (848 mug g(-1)), cadmium (0.4 mug g(-1)), lead (18.7 mug g(-1)), and mercury (0.1 mug g(-1)). As well, goldenseal is an endangered species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II. Therefore, the practice of wild-harvest is actually decimating natural populations of goldenseal and endangering its genetic diversity. In vitro propagation protocol by tissue culture was developed for producing high-quality tissues of goldenseal. Significantly more de novo, regeneration was induced on stem explants of 3-mo.-old plants cultured on a medium containing 10 muM 6-benzylaminopurine (BA) (22 regenerants per explant) than any other treatment. Subculture of the regenerants on a medium devoid of growth regulators resulted in the development of, complete plants that were acclimatized and thrived in standard greenhouse conditions. The plants regenerated in vitro contained the lowest levels of heavy metals. The findings of this study provide the first evidence that heavy metal contaminants bioaccumulate in goldenseal tissues and also provide a method for germplasm conservation, mass multiplication, and production of goldenseal tissues free from abiotic contamination.; Hydrastis canadensis L. (Goldenseal) is an endangered medicinal plant used in the treatment of many ailments, such as gastrointestinal disturbances, urinary disorders, hemorrhage, skin, mouth and eye infections, and inflammation. Commercial preparations of wild-harvested goldenseal were found to contain heavy metal contaminants including aluminum (848 mug g(-1)), cadmium (0.4 mug g(-1)), lead (18.7 mug g(-1)), and mercury (0.1 mug g(-1)). As well, goldenseal is an endangered species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II. Therefore, the practice of wild-harvest is actually decimating natural populations of goldenseal and endangering its genetic diversity. In vitro propagation protocol by tissue culture was developed for producing high-quality tissues of goldenseal. Significantly more de novo, regeneration was induced on stem explants of 3-mo.-old plants cultured on a medium containing 10 muM 6-benzylaminopurine (BA) (22 regenerants per explant) than any other treatment. Subculture of the regenerants on a medium devoid of growth regulators resulted in the development of, complete plants that were acclimatized and thrived in standard greenhouse conditions. The plants regenerated in vitro contained the lowest levels of heavy metals. The findings of this study provide the first evidence that heavy metal contaminants bioaccumulate in goldenseal tissues and also provide a method for germplasm conservation, mass multiplication, and production of goldenseal tissues free from abiotic contamination. |
| Keyword | Hydrastis Canadensis l Medicinal Plant Plant Regeneration Heavy Metal Contamination |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1079/IVP2003499 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | CULTURES |
| WOS Research Area | Plant Sciences ; Cell Biology ; Developmental Biology |
| WOS Subject | Plant Sciences ; Cell Biology ; Developmental Biology |
| WOS ID | WOS:000220431300009 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/4938 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Univ Guelph, Dept Plant Agr, Div Biotechnol, Guelph, ON N1G 2W1, Canada 2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 3.Natl Trop Bot Garden, Inst Ethnobot, Kalaheo, HI USA 4.Univ Notre Dame, Dept Civil Engn & Geol Sci, Notre Dame, IN 46556 USA |
| Recommended Citation GB/T 7714 | Liu, CZ,Murch, SJ,Jain, JC,et al. Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2004,40(1):75-79. |
| APA | Liu, CZ,Murch, SJ,Jain, JC,&Saxena, PK.(2004).Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,40(1),75-79. |
| MLA | Liu, CZ,et al."Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 40.1(2004):75-79. |
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| File Name/Size | DocType | Version | Access | License | ||
| Goldenseal (Hydrasti（162KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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