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Identification of major active constituents in the fingerprint of Salvia miltiorrhiza Bunge developed by high-speed counter-current chromatography
Alternative TitleJ. Chromatogr. A
Gu, M; Zhang, GF; Su, ZG; Fan, OY
2004-07-02
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1041Issue:1-2Pages:239-243
AbstractHigh-speed counter-current chromatography was applied as a method to develop fingerprinting of Salvia miltiorrhiza Bunge, a popular traditional Chinese medicine, in our previous study. Important active constituents that were directly related to the therapy effect should be identified. Each effluent fraction and standard samples (cryptotanshinone, tanshinoneI and tanshinoneIIA) were analyzed by ultraviolet spectroscopy and liquid chromatography-mass spectroscopy. It was concluded from the UV-Vis spectrograms, retention times in LC analysis and mass spectrograms, that fractions 7, 8 and 11 were respectively cryptotanshinone (M-r 296), tanshinone I (M-r 276) and tanshinone IIA (M-r 294). (C) 2004 Elsevier B.V. All rights reserved.; High-speed counter-current chromatography was applied as a method to develop fingerprinting of Salvia miltiorrhiza Bunge, a popular traditional Chinese medicine, in our previous study. Important active constituents that were directly related to the therapy effect should be identified. Each effluent fraction and standard samples (cryptotanshinone, tanshinoneI and tanshinoneIIA) were analyzed by ultraviolet spectroscopy and liquid chromatography-mass spectroscopy. It was concluded from the UV-Vis spectrograms, retention times in LC analysis and mass spectrograms, that fractions 7, 8 and 11 were respectively cryptotanshinone (M-r 296), tanshinone I (M-r 276) and tanshinone IIA (M-r 294). (C) 2004 Elsevier B.V. All rights reserved.
KeywordSalvia Miltiorrhiza Counter-current Chromatography Fingerprinting Pharmaceutical Analysis Cryptotanshinone Tanshinones
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2004.04.030
URL查看原文
Indexed BySCI
Language英语
WOS KeywordLIQUID-CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; PURIFICATION ; DITERPENOIDS ; TANSHINONES ; SEPARATION
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000222284700028
Citation statistics
Cited Times:89[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4946
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Gu, M,Zhang, GF,Su, ZG,et al. Identification of major active constituents in the fingerprint of Salvia miltiorrhiza Bunge developed by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1041(1-2):239-243.
APA Gu, M,Zhang, GF,Su, ZG,&Fan, OY.(2004).Identification of major active constituents in the fingerprint of Salvia miltiorrhiza Bunge developed by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1041(1-2),239-243.
MLA Gu, M,et al."Identification of major active constituents in the fingerprint of Salvia miltiorrhiza Bunge developed by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1041.1-2(2004):239-243.
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