CAS OpenIR  > 研究所(批量导入)
In vitro culture and temporary immersion bioreactor production of Crescentia cujete
Alternative TitlePlant Cell Tissue Organ Cult.
Murch, SJ; Liu, CZ; Romero, RM; Saxena, PK
2004-07-01
Source PublicationPLANT CELL TISSUE AND ORGAN CULTURE
ISSN0167-6857
Volume78Issue:1Pages:63-68
AbstractCrescentia cujete L. is a widely distributed medicinal tree with a diverse range of phytochemicals used as medicinal compounds. Seedlings of wild- harvested C. cujete were established in vitro and used as the starting material for the establishment of axenic cultures. Shoots were proliferated from nodal segments and were maintained over a period of more than 2 years by sequential subculture on a medium containing 1.0 mu mol l(-1) kinetin. De novo regeneration was induced on petiole sections cultured onto a medium containing thidiazuron in combination with 2,4- dichlorophenoxyacetic acid. Axenic cultures were also used to test the efficiency of three different cultivation systems for production of biomass of C. cujete. Growth of plantlets in a temporary immersion bioreactor resulted in significant increases in biomass, leaf number, shoot height and transplant efficiency. Plantlets grown in the bioreactors were acclimatized under greenhouse conditions. Together, these experiments have established optimized parameters for propagation and growth of C. cujete plantlets in a sterile controlled environment for biochemical characterization and production of high- quality medicinal products.; Crescentia cujete L. is a widely distributed medicinal tree with a diverse range of phytochemicals used as medicinal compounds. Seedlings of wild- harvested C. cujete were established in vitro and used as the starting material for the establishment of axenic cultures. Shoots were proliferated from nodal segments and were maintained over a period of more than 2 years by sequential subculture on a medium containing 1.0 mu mol l(-1) kinetin. De novo regeneration was induced on petiole sections cultured onto a medium containing thidiazuron in combination with 2,4- dichlorophenoxyacetic acid. Axenic cultures were also used to test the efficiency of three different cultivation systems for production of biomass of C. cujete. Growth of plantlets in a temporary immersion bioreactor resulted in significant increases in biomass, leaf number, shoot height and transplant efficiency. Plantlets grown in the bioreactors were acclimatized under greenhouse conditions. Together, these experiments have established optimized parameters for propagation and growth of C. cujete plantlets in a sterile controlled environment for biochemical characterization and production of high- quality medicinal products.
KeywordAxenic Cultures Calabash Tree Crescentia Cujete Medicinal Plant Temporary Immersion Bioreactor Thidiazuron
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordINDUCED SOMATIC EMBRYOGENESIS ; X HORTORUM BAILEY ; THIDIAZURON ; MORPHOGENESIS ; REGULATOR ; PEANUT ; GROWTH ; FRUITS ; AUXIN
WOS Research AreaBiotechnology & Applied Microbiology ; Plant Sciences
WOS SubjectBiotechnology & Applied Microbiology ; Plant Sciences
WOS IDWOS:000220303400008
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4951
Collection研究所(批量导入)
Affiliation1.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada
2.Natl Trop Bot Garden, Inst Ethnobot, Kalaheo, HI 96741 USA
3.Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China
4.Univ Costa Rica, Escuela Quim, San Pedro, Costa Rica
5.Inst Nacl Biodiversidad, Santo Domingo Heredia, Costa Rica
Recommended Citation
GB/T 7714
Murch, SJ,Liu, CZ,Romero, RM,et al. In vitro culture and temporary immersion bioreactor production of Crescentia cujete[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2004,78(1):63-68.
APA Murch, SJ,Liu, CZ,Romero, RM,&Saxena, PK.(2004).In vitro culture and temporary immersion bioreactor production of Crescentia cujete.PLANT CELL TISSUE AND ORGAN CULTURE,78(1),63-68.
MLA Murch, SJ,et al."In vitro culture and temporary immersion bioreactor production of Crescentia cujete".PLANT CELL TISSUE AND ORGAN CULTURE 78.1(2004):63-68.
Files in This Item:
File Name/Size DocType Version Access License
In vitro culture and(257KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Murch, SJ]'s Articles
[Liu, CZ]'s Articles
[Romero, RM]'s Articles
Baidu academic
Similar articles in Baidu academic
[Murch, SJ]'s Articles
[Liu, CZ]'s Articles
[Romero, RM]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Murch, SJ]'s Articles
[Liu, CZ]'s Articles
[Romero, RM]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.