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Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system
Wang, Xu-Dong1,2; Hu, Chun-Yan1; Qin, Chao1; Dong, Yue-Sheng1; Ying, Guo-Qing2; Xiu, Zhi-Long1; Su, Zhi-Guo3
2021-07-29
Source PublicationENGINEERING IN LIFE SCIENCES
ISSN1618-0240
Pages10
AbstractClavulanic acid (CA) is usually used together with other beta-lactam antibiotics as combination drugs to inhibit bacterial beta-lactamases, which is mainly produced from the fermentation of microorganism such as Streptomyces clavuligerus. Recently, it is still a challenge for downstream processing of low concentration and unstable CA from fermentation broth with high solid content, high viscosity, and small cell size. In this study, an integrated process was developed for simultaneous solid-liquid separation and primary purification of CA from real fermentation broth of S. clavuligerus using salting-out extraction system (SOES). First, different SOESs were investigated, and a suitable SOES composed of ethanol/phosphate was chosen and further optimized using the pretreated fermentation broth. Then, the optimal system composed of 20% ethanol/15% K2HPO4 and 10% KH2PO4 w/w was used to direct separation of CA from untreated fermentation broth. The result showed that the partition coefficient (K) and recovery yield (Y) of CA from untreated fermentation broth were 29.13 and 96.8%, respectively. Simultaneously, the removal rates of the cells and proteins were 99.8% and 63.3%, respectively. Compared with the traditional method of membrane filtration or liquid-liquid extraction system, this developed SOES showed the advantages of simple operation, shorter operation time, lower process cost and higher recovery yield of CA. These results demonstrated that the developed SOES could be used as an attractive alternative for the downstream processing of CA from real fermentation broth.
Keywordclavulanic acid fermentation broth salting-out extraction solid-liquid separation
DOI10.1002/elsc.202000091
Language英语
WOS KeywordAQUEOUS 2-PHASE SYSTEM ; STABILITY ; 1,3-PROPANEDIOL ; 2,3-BUTANEDIOL
Funding ProjectNational Natural Science Foundation of China[22078042] ; Natural Science Foundation of Zhejiang Province[LY21B060007]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational Natural Science Foundation of China ; Natural Science Foundation of Zhejiang Province
WOS IDWOS:000679128800001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/49517
Collection中国科学院过程工程研究所
Corresponding AuthorXiu, Zhi-Long
Affiliation1.Dalian Univ Technol, Sch Bioengn, 2 Linggong Rd, Dalian 116024, Peoples R China
2.Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou, Zhejiang, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xu-Dong,Hu, Chun-Yan,Qin, Chao,et al. Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system[J]. ENGINEERING IN LIFE SCIENCES,2021:10.
APA Wang, Xu-Dong.,Hu, Chun-Yan.,Qin, Chao.,Dong, Yue-Sheng.,Ying, Guo-Qing.,...&Su, Zhi-Guo.(2021).Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system.ENGINEERING IN LIFE SCIENCES,10.
MLA Wang, Xu-Dong,et al."Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system".ENGINEERING IN LIFE SCIENCES (2021):10.
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