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In vitro protein refolding by chromatographic procedures
Alternative TitleProtein Expr. Purif.
Li, M; Su, ZG; Janson, JC
2004
Source PublicationPROTEIN EXPRESSION AND PURIFICATION
ISSN1046-5928
Volume33Issue:1Pages:1-10
AbstractIn vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results. (C) 2003 Elsevier Inc. All rights reserved.; In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results. (C) 2003 Elsevier Inc. All rights reserved.
KeywordIn Vitro proteIn refoldIng Inclusion Bodies Chromatographic Techniques Gradient Techniques
SubtypeReview
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.pep.2003.08.023
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSIZE-EXCLUSION CHROMATOGRAPHY ; ION-EXCHANGE CHROMATOGRAPHY ; IMMOBILIZED LIPOSOME CHROMATOGRAPHY ; UROKINASE-PLASMINOGEN-ACTIVATOR ; COLI INCLUSION-BODIES ; EGG-WHITE LYSOZYME ; ESCHERICHIA-COLI ; RECOMBINANT PROTEINS ; CARBONIC-ANHYDRASE ; STEP PURIFICATION
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000187587500001
Citation statistics
Cited Times:140[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4952
Collection研究所(批量导入)
Affiliation1.Uppsala Biomed Ctr, Dept Surface Biotechnol, SE-75123 Uppsala, Sweden
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
3.Amersham Biosci R&D Dept, Uppsala, Sweden
Recommended Citation
GB/T 7714
Li, M,Su, ZG,Janson, JC. In vitro protein refolding by chromatographic procedures[J]. PROTEIN EXPRESSION AND PURIFICATION,2004,33(1):1-10.
APA Li, M,Su, ZG,&Janson, JC.(2004).In vitro protein refolding by chromatographic procedures.PROTEIN EXPRESSION AND PURIFICATION,33(1),1-10.
MLA Li, M,et al."In vitro protein refolding by chromatographic procedures".PROTEIN EXPRESSION AND PURIFICATION 33.1(2004):1-10.
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