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Microencapsulation of bovine hemoglobin with high bio-activity and high entrapment efficiency using a W/O/W double emulsion technique
Alternative TitleColloid Surf. B-Biointerfaces
Meng, FT; Ma, GH; Liu, YD; Qiu, W; Su, ZG
2004-02-15
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume33Issue:3-4Pages:177-183
AbstractThis study focused on the influences of solvent removal method and wall polymer composition on microspheres characteristics in W/O/W double emulsion procedure. Monomethoxypoly(ethylene glycol)-b-poly-D,L-lactide (PELA) microspheres containing bovine hemoglobin (BHb, a model protein) were prepared by four solvent removal methods, including solvent-evaporation at atmosphere, at reduced pressure, solvent-extraction and solvent-diffusion methods, where the last method used ethyl acetate (EA) as organic solvent and the others used methylene chloride (MC). The bio-activity of encapsulated BHb, encapsulation efficiency, particle size and surface morphology of microspheres were evaluated in relation to the influences of solvent removal method and PELA composition. BHb encapsulated by the W/O/W double emulsion-sol vent diffusion method with EA as organic solvent displayed a bio-activity near to that of native BHb. The efficiency of BHb entrapment achieved by this method was much higher than those by other methods (ca. 90% versus 30%). When using this process, the copolymers with MPEG 2000 block (molecular weight of PEG block: 2000 g/mol) yielded much higher efficiencies of BHb entrapment than those with MPEG 5000 block (90% versus 36%). Copolymer composition had less impact on microsphere size, but had a pronounced effect on surface morphology of microspheres. This study suggests that the W/O/W double emulsion-solvent diffusion method with EA as organic solvent is an effective process to prepare microspheres containing therapeutic proteins, and that the PELA copolymers containing MPEG 2000 block are promising wall material for biodegradable microsphere protein delivery system. (C) 2003 Elsevier B.V. All rights reserved.; This study focused on the influences of solvent removal method and wall polymer composition on microspheres characteristics in W/O/W double emulsion procedure. Monomethoxypoly(ethylene glycol)-b-poly-D,L-lactide (PELA) microspheres containing bovine hemoglobin (BHb, a model protein) were prepared by four solvent removal methods, including solvent-evaporation at atmosphere, at reduced pressure, solvent-extraction and solvent-diffusion methods, where the last method used ethyl acetate (EA) as organic solvent and the others used methylene chloride (MC). The bio-activity of encapsulated BHb, encapsulation efficiency, particle size and surface morphology of microspheres were evaluated in relation to the influences of solvent removal method and PELA composition. BHb encapsulated by the W/O/W double emulsion-sol vent diffusion method with EA as organic solvent displayed a bio-activity near to that of native BHb. The efficiency of BHb entrapment achieved by this method was much higher than those by other methods (ca. 90% versus 30%). When using this process, the copolymers with MPEG 2000 block (molecular weight of PEG block: 2000 g/mol) yielded much higher efficiencies of BHb entrapment than those with MPEG 5000 block (90% versus 36%). Copolymer composition had less impact on microsphere size, but had a pronounced effect on surface morphology of microspheres. This study suggests that the W/O/W double emulsion-solvent diffusion method with EA as organic solvent is an effective process to prepare microspheres containing therapeutic proteins, and that the PELA copolymers containing MPEG 2000 block are promising wall material for biodegradable microsphere protein delivery system. (C) 2003 Elsevier B.V. All rights reserved.
KeywordBiodegradable Microspheres Monomethoxypoly(Ethylene Glycol)-b-poly-d Bovine Hemoglobin L-lactide Stability Protein Entrapment Efficiency
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
DOI10.1016/j.colsurfb.2003.10.003
URL查看原文
Indexed BySCI
Language英语
WOS KeywordBIODEGRADABLE MICROSPHERES ; GLYCOL) MICROSPHERES ; ENCAPSULATION ; LYSOZYME ; PROTEIN ; MICROPARTICLES ; INTERFACE ; STABILITY ; CHLORIDE
WOS Research AreaBiophysics ; Chemistry ; Materials Science
WOS SubjectBiophysics ; Chemistry, Physical ; Materials Science, Biomaterials
WOS IDWOS:000189085900005
Citation statistics
Cited Times:76[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4960
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Meng, FT,Ma, GH,Liu, YD,et al. Microencapsulation of bovine hemoglobin with high bio-activity and high entrapment efficiency using a W/O/W double emulsion technique[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2004,33(3-4):177-183.
APA Meng, FT,Ma, GH,Liu, YD,Qiu, W,&Su, ZG.(2004).Microencapsulation of bovine hemoglobin with high bio-activity and high entrapment efficiency using a W/O/W double emulsion technique.COLLOIDS AND SURFACES B-BIOINTERFACES,33(3-4),177-183.
MLA Meng, FT,et al."Microencapsulation of bovine hemoglobin with high bio-activity and high entrapment efficiency using a W/O/W double emulsion technique".COLLOIDS AND SURFACES B-BIOINTERFACES 33.3-4(2004):177-183.
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