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Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments
Zhou, Jiemin1; Xing, Jianmin2
2021-08-01
Source PublicationWATER RESEARCH
ISSN0043-1354
Volume201Pages:8
AbstractAs bridge in global cycles of carbon, nitrogen, and sulfur, sulfate-reducing bacteria (SRB) play more and more important role under various environments, especially the saline-alkali environments with significant increase in area caused by human activities. Sulfate reduction can be inhibited by environmental nitrate. However, how SRB cope with environmental nitrate stress in these extreme environments still remain unclear. Here, after a longterm enrichment of sediment from saline-alkali Qinghai Lake of China using anaerobic filter reactors, nitrate was added to evaluate the response of SRB. With the increase in nitrate concentrations, the inhibition on sulfate reduction was gradually observed. Interestingly, extension of hydraulic retention time can relieve the inhibition caused by high nitrate concentration. Mass balance analysis showed that nitrate reduction is prior to sulfate reduction. Further metatranscriptomic analysis shows that, genes of nitrite reductase (periplasmic cytochrome c nitrite reductase gene) and energy metabolisms (lactate dehydrogenase, formate dehydrogenase, pyruvate: ferredoxin-oxidoreductase, and fumarate reductase genes) in SRB was down-regulated, challenging the longheld opinion that up-regulation of these genes can relieve the nitrate inhibition. Most importantly, the nitrate addition activated the denitrification pathway in denitrifying bacteria (DB) via significantly up-regulating the expression of the corresponding genes (nitrite reductase, nitric oxide reductase c subunit, nitric oxide reductase activation protein and nitrous oxide reductase genes), quickly reducing the environmental nitrate and relieving the nitrate inhibition on SRB. Our findings unravel that in response to environmental nitrate stress, haloalkaliphilic SRB show dependency on DB, and expand our knowledge of microbial relationship during sulfur and nitrogen cycles.
KeywordSulfate-reducing bacteria Denitrifying bacteria Metatranscriptomic analysis Nitrate inhibition Enriched biomass
DOI10.1016/j.watres.2021.117354
Language英语
WOS KeywordDESULFOVIBRIO-VULGARIS HILDENBOROUGH ; GENE-EXPRESSION ANALYSIS ; ELECTRON-DONORS ; RNA-SEQ ; PERFORMANCE ; INHIBITION ; STRESS ; TRANSCRIPTOMES ; CONSEQUENCES ; COMMUNITIES
Funding ProjectKey Laboratory of Drinking Water Science and Technology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences[19Z02KLDWST]
WOS Research AreaEngineering ; Environmental Sciences & Ecology ; Water Resources
WOS SubjectEngineering, Environmental ; Environmental Sciences ; Water Resources
Funding OrganizationKey Laboratory of Drinking Water Science and Technology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences
WOS IDWOS:000681702200007
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/49609
Collection中国科学院过程工程研究所
Corresponding AuthorZhou, Jiemin
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Jiemin,Xing, Jianmin. Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments[J]. WATER RESEARCH,2021,201:8.
APA Zhou, Jiemin,&Xing, Jianmin.(2021).Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments.WATER RESEARCH,201,8.
MLA Zhou, Jiemin,et al."Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments".WATER RESEARCH 201(2021):8.
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