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Polyethylene glycol increases purification and recovery, alters retention behavior in flow-through chromatography of hemoglobin
Alternative TitleJ. Chromatogr. A
Lu, XL; Zhao, DX; Ma, GH; Su, ZG
2004-12-03
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1059Issue:1-2Pages:233-237
AbstractFlow-through chromatography was a method for purification of hemoglobin (Hb) from red cell lysate. The presence of 0-5% polyethylene glycol (PEG) increased the retention time of Hb peak from 15 min to 20 min in flow-through ion-exchange chromatography (IEC) but decreased the retention time from 88 min to 62 min in the hydrophobic interaction chromatography (HIC). However, the purification and the recovery were both increased. For IEC the recovery of hemoglobin increased from 75% to more than 90%, and the purified Hb showed single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and one peak in size-exclusion HPLC. For HIC, the recovery of hemoglobin was improved significantly from 20% to 85% and the removal of lipids was 100%. The bioactivity of hemoglobin was well preserved in these two chromatographic processes. The mechanism for the effect of PEG in these two flow-through chromatographic processes was discussed. (C) 2004 Elsevier B.V. All rights reserved.; Flow-through chromatography was a method for purification of hemoglobin (Hb) from red cell lysate. The presence of 0-5% polyethylene glycol (PEG) increased the retention time of Hb peak from 15 min to 20 min in flow-through ion-exchange chromatography (IEC) but decreased the retention time from 88 min to 62 min in the hydrophobic interaction chromatography (HIC). However, the purification and the recovery were both increased. For IEC the recovery of hemoglobin increased from 75% to more than 90%, and the purified Hb showed single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and one peak in size-exclusion HPLC. For HIC, the recovery of hemoglobin was improved significantly from 20% to 85% and the removal of lipids was 100%. The bioactivity of hemoglobin was well preserved in these two chromatographic processes. The mechanism for the effect of PEG in these two flow-through chromatographic processes was discussed. (C) 2004 Elsevier B.V. All rights reserved.
KeywordIon-exchange Chromatography Hemoglobin Hydrophobic Interaction Chromatography Polyethylene Glycol Purification
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2004.10.047
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSUBSTITUTES
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000225412200030
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4986
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Univ Sci & Technol Beijing, Beijing 100070, Peoples R China
Recommended Citation
GB/T 7714
Lu, XL,Zhao, DX,Ma, GH,et al. Polyethylene glycol increases purification and recovery, alters retention behavior in flow-through chromatography of hemoglobin[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1059(1-2):233-237.
APA Lu, XL,Zhao, DX,Ma, GH,&Su, ZG.(2004).Polyethylene glycol increases purification and recovery, alters retention behavior in flow-through chromatography of hemoglobin.JOURNAL OF CHROMATOGRAPHY A,1059(1-2),233-237.
MLA Lu, XL,et al."Polyethylene glycol increases purification and recovery, alters retention behavior in flow-through chromatography of hemoglobin".JOURNAL OF CHROMATOGRAPHY A 1059.1-2(2004):233-237.
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