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| An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity | |
| Su, Ziran1; Luo, Jianquan2; Sigurdardottir, Sigyn Bjork1; Manferrari, Thomas1; Jankowska, Katarzyna1,3; Pinelo, Manuel1 | |
| 2021-11-01 | |
| Source Publication | CARBOHYDRATE POLYMERS
 |
| ISSN | 0144-8617 |
| Volume | 271Pages:10 |
| Abstract | An enzymatic membrane reactor (EMR) with immobilized dextranase provides an excellent opportunity for tailoring the molecular weight (Mw) of oligodextran to significantly improve product quality. However, a highly efficient EMR for oligodextran production is still lacking and the effect of enzyme immobilization strategy on dextranase hydrolysis behavior has not been studied yet. In this work, a functional layer of polydopamine (PDA) or nanoparticles made of tannic acid (TA) and hydrolysable 3-amino-propyltriethoxysilane (APTES) was first coated on commercial membranes. Then cross-linked dextranase or non-cross-linked dextranase was loaded onto the modified membranes using incubation mode or fouling-induced mode. The fouling-induced mode was a promising enzyme immobilization strategy on the membrane surface due to its higher enzyme loading and activity. Moreover, unlike the non-cross-linked dextranase that exhibited a normal endo-hydrolysis pattern, we surprisingly found that the cross-linked dextranase loaded on the PDA modified surface exerted an exo-hydrolysis pattern, possibly due to mass transfer limitations. Such alteration of hydrolysis pattern has rarely been reported before. Based on the hydrolysis behavior of the immobilized dextranase in different EMRs, we propose potential applications for the oligodextran products. This study presents a unique perspective on the relation between the enzyme immobilization process and the immobilized enzyme hydrolysis behavior, and thus opens up a variety of possibilities for the design of a high-performance EMR. |
| Keyword | Enzymatic membrane reactor Enzyme immobilization Dextranase Oligodextran Biocatalytic membrane |
| DOI | 10.1016/j.carbpol.2021.118430 |
| Language | 英语 |
| WOS Keyword | INTERFACIAL INTERACTIONS ; SURFACE ; GLUTARALDEHYDE ; CHALLENGES ; FILTRATION ; STABILITY ; BEHAVIOR ; DESIGN ; IMPACT ; CLEAS |
| Funding Project | Novo Nordisk Fonden[NNF19OC0057684] ; China Scholarship Council[201804910747] ; Poznan University of Technology, Poland |
| WOS Research Area | Chemistry ; Polymer Science |
| WOS Subject | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| Funding Organization | Novo Nordisk Fonden ; China Scholarship Council ; Poznan University of Technology, Poland |
| WOS ID | WOS:000685091700006 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/49908 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Luo, Jianquan; Pinelo, Manuel |
| Affiliation | 1.Tech Univ Denmark, Proc & Syst Engn Ctr, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark 2.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Poznan Univ Tech, Fac Chem Technol, Inst Chem Technol & Engn, Berdychowo 4, PL-60965 Poznan, Poland |
| Recommended Citation GB/T 7714 | Su, Ziran,Luo, Jianquan,Sigurdardottir, Sigyn Bjork,et al. An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity[J]. CARBOHYDRATE POLYMERS,2021,271:10. |
| APA | Su, Ziran,Luo, Jianquan,Sigurdardottir, Sigyn Bjork,Manferrari, Thomas,Jankowska, Katarzyna,&Pinelo, Manuel.(2021).An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity.CARBOHYDRATE POLYMERS,271,10. |
| MLA | Su, Ziran,et al."An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity".CARBOHYDRATE POLYMERS 271(2021):10. |
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