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Preparation of superparamagnetic immunomicrospheres and application for antibody purification
Alternative TitleJ. Appl. Polym. Sci.
Liu, XQ; Guan, YP; Yang, Y; Ma, ZY; Wu, XB; Liu, HZ
2004-12-05
Source PublicationJOURNAL OF APPLIED POLYMER SCIENCE
ISSN0021-8995
Volume94Issue:5Pages:2205-2211
AbstractA novel and effective protocol for the preparation of superparamagnetic immunomicrospheres has been developed. First, micro-size magnetic poly (methacrylate-divinylbenzene) (PMA-DVB) spheres were prepared by a modified suspension polymerization method. The oleic acid coated magnetite (Fe3O4,) nanoparticles made by coprecipitation were mixed with monomers of MA, DVB, and initiator benzoyl peroxide (BPO) to form oil in water emulsion droplets with the presence of poly (vinyl alcohol) (PVA1788) as a stabilizer. The polymerization reaction was carried out in a 2-L beaker equipped with four vertical stainless steel baffleplates by increasing the temperature of the mixture at a controlled rate. The resulting magnetic microspheres are micro-sized (less than 8mum in diameter) and 80 percent of them are in the size ranging from 1 to 5 mum. Then, they were highly functionalized via ammonolysis reaction with ethylenediamine, and the surface amino-modified magnetic microspheres were obtained. The morphology and properties of these magnetic microspheres were examined by SEM, TEM, VSM, and FT-IR. Affinity ligand protein A (ProtA) was covalently immobilized to the amino-modified magnetic microspheres by the glutaraldehyde method. These ProtA-immobilized magnetic immunomicrospheres were effective for affinity bioseparation processes, as was demonstrated by the efficient immunoaffinity purification of antibodies IgG2a (22mg per gram of microspheres) from mouse ascites.(C) 2004 Wiley Periodicals, Inc.; A novel and effective protocol for the preparation of superparamagnetic immunomicrospheres has been developed. First, micro-size magnetic poly (methacrylate-divinylbenzene) (PMA-DVB) spheres were prepared by a modified suspension polymerization method. The oleic acid coated magnetite (Fe3O4,) nanoparticles made by coprecipitation were mixed with monomers of MA, DVB, and initiator benzoyl peroxide (BPO) to form oil in water emulsion droplets with the presence of poly (vinyl alcohol) (PVA1788) as a stabilizer. The polymerization reaction was carried out in a 2-L beaker equipped with four vertical stainless steel baffleplates by increasing the temperature of the mixture at a controlled rate. The resulting magnetic microspheres are micro-sized (less than 8mum in diameter) and 80 percent of them are in the size ranging from 1 to 5 mum. Then, they were highly functionalized via ammonolysis reaction with ethylenediamine, and the surface amino-modified magnetic microspheres were obtained. The morphology and properties of these magnetic microspheres were examined by SEM, TEM, VSM, and FT-IR. Affinity ligand protein A (ProtA) was covalently immobilized to the amino-modified magnetic microspheres by the glutaraldehyde method. These ProtA-immobilized magnetic immunomicrospheres were effective for affinity bioseparation processes, as was demonstrated by the efficient immunoaffinity purification of antibodies IgG2a (22mg per gram of microspheres) from mouse ascites.(C) 2004 Wiley Periodicals, Inc.
KeywordSynthesis Magnetic Microspheres Copolymerization Antibody Purification Bioengineering
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/app.21168
URL查看原文
Indexed BySCI
Language英语
WOS KeywordA AFFINITY-CHROMATOGRAPHY ; PROTEIN-A ; SUSPENSION POLYMERIZATION ; MAGNETIC PARTICLES ; MICROSPHERES ; SEPARATIONS ; STYRENE ; BEADS ; CELLS
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
WOS IDWOS:000224759700045
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/4998
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, XQ,Guan, YP,Yang, Y,et al. Preparation of superparamagnetic immunomicrospheres and application for antibody purification[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2004,94(5):2205-2211.
APA Liu, XQ,Guan, YP,Yang, Y,Ma, ZY,Wu, XB,&Liu, HZ.(2004).Preparation of superparamagnetic immunomicrospheres and application for antibody purification.JOURNAL OF APPLIED POLYMER SCIENCE,94(5),2205-2211.
MLA Liu, XQ,et al."Preparation of superparamagnetic immunomicrospheres and application for antibody purification".JOURNAL OF APPLIED POLYMER SCIENCE 94.5(2004):2205-2211.
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