CAS OpenIR
Wide-Range, Rapid, and Specific Identification of Pathogenic Bacteria by Surface-Enhanced Raman Spectroscopy
Liu, Siying1,2,3,4; Hu, Qiushi5; Li, Chao6; Zhang, Fangrong7,8; Gu, Hongjing9; Wang, Xinrui10; Li, Shuang5; Xue, Lei5; Madl, Tobias1,2,3,7,8; Zhang, Yun1,2,3,4; Zhou, Lei5
2021-08-27
Source PublicationACS SENSORS
ISSN2379-3694
Volume6Issue:8Pages:2911-2919
AbstractSensitive, selective, rapid, and label-free detection of pathogenic bacteria with high generality is of great importance for clinical diagnosis, biosecurity, and public health. However, most traditional approaches, such as microbial cultures, are time-consuming and laborious. To circumvent these problems, surface-enhanced Raman spectroscopy (SERS) appears to be a powerful technique to characterize bacteria at the single-cell level. Here, by SERS, we report a strategy for the rapid and specific detection of 22 strains of common pathogenic bacteria. A novel and high-quality silver nanorod SERS substrate, prepared by the facile interface self-assembly method, was utilized to acquire the chemical fingerprint information of pathogens with improved sensitivity. We also applied the mathematical analysis methods, such as the t-test and receiver operating characteristic method, to determine the Raman features of these 22 strains and demonstrate the clear identification of most bacteria (20 strains) from the rest and also the reliability of this SERS sensor. This rapid and specific strategy for wide-range bacterial detection offers significant advantages over existing approaches and sets the base for automated and onsite detection of pathogenic bacteria in a complex real-life situation.
KeywordAgNR substrate bacterial diagnostics label-free pathogenic bacteria receiver operating characteristic analysis surface-enhanced Raman spectroscopy (SERS)
DOI10.1021/acssensors.1c00641
Language英语
WOS KeywordCRYPTOCOCCUS-NEOFORMANS ; SCATTERING ; GROWTH ; SERS ; NANOPARTICLES
Funding ProjectNational Key Research and Development Program[2019YFC1606600] ; National Key Research and Development Program[2019YFC1606602] ; Beijing Nova Program[Z191100001119005] ; Innovation Program of National Defense Science and Technology[18-163-12-ZT-005-01601] ; Fujian Provincial Development and Reform Commission 2018 Strategic Emerging Industry Key Areas, Development and Reform Investment [2018][381] ; National Natural Science Foundation of China[21773246] ; National Natural Science Foundation of China[21403176] ; Natural Science Foundation of Fujian Province[2019J01123] ; Austrian Science Foundation[P28854] ; Austrian Science Foundation[I3792] ; Austrian Science Foundation[DK-MCD W1226] ; Integrative Metabolism Research Center Graz ; Styrian government (Zukunftsfonds) ; BioTechMed/Graz (Flagship project) ; Austrian infra-structure program 2016/2017 ; Austrian Research Promotion Agency[FFG: 864690] ; Austrian Research Promotion Agency[870454]
WOS Research AreaChemistry ; Science & Technology - Other Topics
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology
Funding OrganizationNational Key Research and Development Program ; Beijing Nova Program ; Innovation Program of National Defense Science and Technology ; Fujian Provincial Development and Reform Commission 2018 Strategic Emerging Industry Key Areas, Development and Reform Investment [2018] ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Austrian Science Foundation ; Integrative Metabolism Research Center Graz ; Styrian government (Zukunftsfonds) ; BioTechMed/Graz (Flagship project) ; Austrian infra-structure program 2016/2017 ; Austrian Research Promotion Agency
WOS IDWOS:000692037700015
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/50092
Collection中国科学院过程工程研究所
Corresponding AuthorMadl, Tobias; Zhang, Yun; Zhou, Lei
Affiliation1.Chinese Acad Sci, Fujian Inst Res Struct Matter, CAS Key Lab Design & Assembly Funct Nanostruct, Fuzhou 350002, Peoples R China
2.Chinese Acad Sci, Fujian Inst Res Struct Matter, Fujian Prov Key Lab Nanomat, Fuzhou 350002, Peoples R China
3.Chinese Acad Sci, Xiamen Inst Rare Earth Mat, Dept Translat Med, Xiamen 361021, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China
6.Acad Mil Med Sci, Inst Med Equipment, Tianjin 300161, Peoples R China
7.Med Univ Graz, Gottfried Schatz Res Ctr Cell Signaling Metab & A, Inst Mol Biol & Biochem, A-8010 Graz, Austria
8.BioTechMed Graz, Graz, Austria
9.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
10.Antiplague Inst Hebei Prov, Zhangjiakou 075000, Peoples R China
Recommended Citation
GB/T 7714
Liu, Siying,Hu, Qiushi,Li, Chao,et al. Wide-Range, Rapid, and Specific Identification of Pathogenic Bacteria by Surface-Enhanced Raman Spectroscopy[J]. ACS SENSORS,2021,6(8):2911-2919.
APA Liu, Siying.,Hu, Qiushi.,Li, Chao.,Zhang, Fangrong.,Gu, Hongjing.,...&Zhou, Lei.(2021).Wide-Range, Rapid, and Specific Identification of Pathogenic Bacteria by Surface-Enhanced Raman Spectroscopy.ACS SENSORS,6(8),2911-2919.
MLA Liu, Siying,et al."Wide-Range, Rapid, and Specific Identification of Pathogenic Bacteria by Surface-Enhanced Raman Spectroscopy".ACS SENSORS 6.8(2021):2911-2919.
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