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Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study
Alternative TitleJ. Chromatogr. A
Lou, XW; Zhu, QS; Lei, Z; van Dongen, JLJ; Meijer, EW
2004-03-12
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1029Issue:1-2Pages:67-75
AbstractA simple chromatographic model was used to simulate the retention behavior of supramolecular complex in size exclusion chromatography (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to investigate the influence of various parameters in the SEC characterization/confirmation of supramolecular association. Peak tailing, peak fronting, peak splitting and retention time shifting were observed under different conditions. It was found that the chromatographic peak shape and retention time were strongly affected by the association constant, sample concentration, as well as the addition of a reactant in the mobile phase. Furthermore, using the same model, the chromatographic process of Hummel-Dreyer procedure was simulated, and the results indicated that the procedure can be a good method for the determination of association constants for AB type complexes. (C) 2004 Elsevier B.V. All rights reserved.; A simple chromatographic model was used to simulate the retention behavior of supramolecular complex in size exclusion chromatography (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to investigate the influence of various parameters in the SEC characterization/confirmation of supramolecular association. Peak tailing, peak fronting, peak splitting and retention time shifting were observed under different conditions. It was found that the chromatographic peak shape and retention time were strongly affected by the association constant, sample concentration, as well as the addition of a reactant in the mobile phase. Furthermore, using the same model, the chromatographic process of Hummel-Dreyer procedure was simulated, and the results indicated that the procedure can be a good method for the determination of association constants for AB type complexes. (C) 2004 Elsevier B.V. All rights reserved.
KeywordSimulation Supramolecular Complex Retention Behavior Association Constant Size Exclusion Chromatography
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2003.12.023
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPROTEIN-BINDING ; GEL-FILTRATION ; SMALL-ZONE
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000189076000009
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5017
Collection研究所(批量导入)
Affiliation1.Tech Univ Eindhoven, Lab Macromol & Organ Chem, NL-5600 MB Eindhoven, Netherlands
2.Chinese Acad Sci, Inst Proc Engn, Multiphase React Lab, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Lou, XW,Zhu, QS,Lei, Z,et al. Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1029(1-2):67-75.
APA Lou, XW,Zhu, QS,Lei, Z,van Dongen, JLJ,&Meijer, EW.(2004).Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study.JOURNAL OF CHROMATOGRAPHY A,1029(1-2),67-75.
MLA Lou, XW,et al."Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study".JOURNAL OF CHROMATOGRAPHY A 1029.1-2(2004):67-75.
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