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Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation
Jia, Yunpu1,2; Samak, Nadia A.1,2,3,5; Hao, Xuemi1,2; Chen, Zheng1,2; Yang, Gama1,2; Zhao, Xuhao1; Mu, Tingzhen1; Yang, Maohua1; Xing, Jianmin1,2,4
2021-12-01
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume176Pages:8
AbstractPET hydmlase (PETase), discovered in Ideonella sakaiensis, is a promising agent for the biodegradation of polyethylene terephthalate (PET) capable of PET decomposition under mild reaction conditions with limited stability and productivity. Here, the immobilization of His-tagged PETase was achieved by synthesizing enzyme-inorganic nanoflowers, PETase@Co-3(PO4)(2), which was designed based on the principle of biomimetic mineralization. Immobilization of PETase onto nanostructured Co-3(PO4)(2) enjoys high enzyme loading and low mass transfer inhibition due to large specific surface area, high movement speed, and large surface curvature caused by small particle size. The nano-effect of inorganic carriers materialize the 10 degrees C optimum temperature swelling of the immobilized PETase with enhanced pH tolerance (6.0-10.0) than the free counterpart. The long-duration reaction showed that the productivity of terephthalic acid (TPA) was 3.5 times higher than that of the free enzyme. PETase@Co-3(PO4)(2) still retained 75% of the initial activity after 12 days compared with the free enzymes, which showed almost no activity. The excellent and stable catalytic performance of PETase@Co-3(PO4)( )2 with low cost demonstrates the synthetical usefulness of immobilization via biomimetic mineralization in the enzyme utilization in industrial PET depolymerization.
KeywordPlastic biodegradation Immobilization of biocatalyst Biomimetic mineralization
DOI10.1016/j.bej.2021.108205
Language英语
WOS KeywordBIOMIMETIC MINERALIZATION ; NANOFLOWERS ; HYDROLASE ; CUTINASE ; BINDING
Funding ProjectNational Natural Science Foundation of China[31961133017] ; National Natural Science Foundation of China[31961133018] ; National Natural Science Foundation of China[31961133019] ; European Union's Horizon 2020 research and innovation program[870294]
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China ; European Union's Horizon 2020 research and innovation program
WOS IDWOS:000697108600031
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/50261
Collection中国科学院过程工程研究所
Corresponding AuthorXing, Jianmin
Affiliation1.Chinese Acad Sci, Inst Proc Engn, CAS Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Coll Chem Engn, Beijing 100049, Peoples R China
3.Univ Duisburg Essen, Aquat Microbiol, Environm Microbiol & Biotechnol, D-4141 Essen, Germany
4.Chem & Chem Engn Guangdong Lab, Shantou 515031, Peoples R China
5.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt
Recommended Citation
GB/T 7714
Jia, Yunpu,Samak, Nadia A.,Hao, Xuemi,et al. Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation[J]. BIOCHEMICAL ENGINEERING JOURNAL,2021,176:8.
APA Jia, Yunpu.,Samak, Nadia A..,Hao, Xuemi.,Chen, Zheng.,Yang, Gama.,...&Xing, Jianmin.(2021).Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation.BIOCHEMICAL ENGINEERING JOURNAL,176,8.
MLA Jia, Yunpu,et al."Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation".BIOCHEMICAL ENGINEERING JOURNAL 176(2021):8.
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