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Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization
Alternative TitleColloid Surf. A-Physicochem. Eng. Asp.
Liu, XQ; Xing, JM; Guan, YP; Shan, GB; Liu, HZ
2004-05-04
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
Volume238Issue:1-3Pages:127-131
AbstractA novel method to synthesize amino-silane modified magnetic silica supports was reported for affinity separation. The magnetite (Fe3O4) nanoparticles were prepared by the coprecipitation of ferrous and ferric salts with NH4OH, and then their surface was coated with silica in sodium silicate solution by acidifying technology to form well-dispersed magnetic silica nanospheres. These magnetic silica nanospheres are of about 50-80 nm in diameter and exhibit superparamagnetic characteristics. The surface of these nanospheres was with amino-silane coupling agent for their attachment to affinity ligands. In this work, bovine serum albumin (BSA) was covalently immobilized onto the amino-silane modified magnetic silica supports by the glutaraldehyde method. The influence of pH, ionic strength as well as the initial protein concentration on BSA immobilization has been studied. The result shows that such amino-silane modified magnetic silica is a well-dispersed and effective superparamagnetic support for bioseparation and the maximum BSA immobilization capacity (up to 86 mg/g) was obtained in 0.1 M phosphate buffer at pH 5.0. (C) 2004 Elsevier B.V. All rights reserved.; A novel method to synthesize amino-silane modified magnetic silica supports was reported for affinity separation. The magnetite (Fe3O4) nanoparticles were prepared by the coprecipitation of ferrous and ferric salts with NH4OH, and then their surface was coated with silica in sodium silicate solution by acidifying technology to form well-dispersed magnetic silica nanospheres. These magnetic silica nanospheres are of about 50-80 nm in diameter and exhibit superparamagnetic characteristics. The surface of these nanospheres was with amino-silane coupling agent for their attachment to affinity ligands. In this work, bovine serum albumin (BSA) was covalently immobilized onto the amino-silane modified magnetic silica supports by the glutaraldehyde method. The influence of pH, ionic strength as well as the initial protein concentration on BSA immobilization has been studied. The result shows that such amino-silane modified magnetic silica is a well-dispersed and effective superparamagnetic support for bioseparation and the maximum BSA immobilization capacity (up to 86 mg/g) was obtained in 0.1 M phosphate buffer at pH 5.0. (C) 2004 Elsevier B.V. All rights reserved.
KeywordMagnetic Supports Amino-silane Protein Immobilization Bioseparation
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.colsurfa.2004.03.004
URL查看原文
Indexed BySCI
Language英语
WOS KeywordFINE MAGNETIC PARTICLES ; MICROEMULSION ; PURIFICATION ; MICROSPHERES ; ADSORPTION ; ENZYMES
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
WOS IDWOS:000222077400016
Citation statistics
Cited Times:129[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5029
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liu, XQ,Xing, JM,Guan, YP,et al. Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2004,238(1-3):127-131.
APA Liu, XQ,Xing, JM,Guan, YP,Shan, GB,&Liu, HZ.(2004).Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,238(1-3),127-131.
MLA Liu, XQ,et al."Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 238.1-3(2004):127-131.
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