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Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide
Li, Haitao1,2; Li, Yuping1; Cao, Hongbin1; Li, Xingang2; Zhang, Yi1; Li, YP
2011-03-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume83Issue:2Pages:124-130
AbstractA novel horseradish peroxidase (HRP)-catalyzed H(2)O(2) process in the presence of reduced nicotinamide adenine dinucleotide (NADH) was applied to remove aqueous pentachlorophenol (PCP). Parameters (PH, H(2)O(2) concentration, HRP activity and NADH dosage) on PCP removal were investigated. It was found that initial 0.05 mM PCP was removed by 98% in HRP-NADH-H(2)O(2) system at pH 5.0 and 30 degrees C for 1 h. Addition of O(2) in HRP-NADH-H(2)O(2) system enhanced the removal rate of PCP due to promoting hydroxyl radicals (center dot OH) and superoxide anion radical) generation, which were confirmed by electron paramagnetic resonance (EPR)-spin trapping method. PCP removal efficiency decreased when center dot O ((2)) over bar and H(2)O(2) were scavenged by superoxide dismutase and catalase in HRP-NADH-O(2) system, indicating that center dot OH/center dot O ((2)) over bar played a great role in the degradation of PCP. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that octachlorinated dibenzodioxin (OCDD) in residual solution was reduced after treated by the HRP-NADH-O(2) process, resulting in lower toxicity of treated solution than conventional enzymatic process. Two enzymatic-catalysis pathways were proposed for PCP removal in HRP-NADH-H(2)O(2)/O(2) system: (i) center dot OH/center dot O ((2)) over bar free radical oxidation (ii) conventional phenoxy polymerization. (C) 2011 Elsevier Ltd. All rights reserved.
KeywordHorseradish Peroxidase Pentachlorophenol Hydroxyl Radicals Superoxide Anion Radical Electron Paramagnetic Resonance Nadh
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.chemosphere.2011.01.014
Indexed BySCI
Language英语
WOS KeywordGENERATED HYDROXYL RADICALS ; DIBENZO-P-DIOXINS ; HORSERADISH-PEROXIDASE ; HYDROGEN-PEROXIDE ; AQUEOUS PENTACHLOROPHENOL ; OXIDATION ; REMOVAL ; ENZYME ; PCP ; PHENOLS
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000288926400005
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5064
Collection湿法冶金清洁生产技术国家工程实验室
Corresponding AuthorLi, YP
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
2.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Li, Haitao,Li, Yuping,Cao, Hongbin,et al. Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide[J]. CHEMOSPHERE,2011,83(2):124-130.
APA Li, Haitao,Li, Yuping,Cao, Hongbin,Li, Xingang,Zhang, Yi,&Li, YP.(2011).Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide.CHEMOSPHERE,83(2),124-130.
MLA Li, Haitao,et al."Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide".CHEMOSPHERE 83.2(2011):124-130.
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