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| Conjugation of Zika virus EDIII with CRM197, 8-arm PEG and mannan for development of an effective Zika virus vaccine | |
He, Yunxia1,2; Yu, Weili1 ; Xiao, Lucheng1,2; Shen, Lijuan1; Qi, Jinming1; Hu, Tao1
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| 2021-11-01 | |
| Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| Volume | 190Pages:713-721 |
| Abstract | Zika virus (ZIKV) induces neurological and autoimmune complications such as microcephaly and Guillain-Barre syndrome. Effective vaccines are necessary to prevent the ZIKV infection. E protein of ZIKV is responsible for virus attachment, entry, and fusion. The domain III of E protein (EDIII) contains the neutralizing epitopes and is ideal to act as an antigen for ZIKV vaccine. However, EDIII is poorly immunogenic. CRM197 is a carrier protein and can activate T helper cells for EDIII. Mannan is a ligand of TLR-4 or TLR-2. Eight-arm PEG can link multiple EDIII molecules in one entity. In the present study, EDIII was covalently conjugated with CRM197, 8-arm PEG and mannan to improve the immunogenicity of EDIII. The conjugate (CRM-EDIII-PM) elicited high EDIII-specific antibody titers in the BALB/c mice. Th1-type cytokines (IFN-gamma and IL-2) and Th2-type cytokines (IL-5 and IL10) were secreted at a marked level. Thus, CRM-EDIII-PM could stimulate potent humoral and cellular immune response to EDIII. The serum exposure of CRM-EDIII-PM to the immune system was prolonged. Moreover, CRM-EDIII-PM did not lead to apparent toxicity to the organs. Therefore, CRM-EDIII-PM was expected as a promising vaccine candidate for its ability to induce strong immune responses. |
| Keyword | Zika virus EDIII Adjuvant |
| DOI | 10.1016/j.ijbiomac.2021.08.177 |
| Language | 英语 |
| WOS Keyword | IMMUNOGENICITY ; IMPROVES ; SAFETY |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS ID | WOS:000710145500008 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/50661 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Qi, Jinming; Hu, Tao |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China |
| First Author Affilication | Center of lonic Liquids and Green Engineering |
| Corresponding Author Affilication | Center of lonic Liquids and Green Engineering |
| Recommended Citation GB/T 7714 | He, Yunxia,Yu, Weili,Xiao, Lucheng,et al. Conjugation of Zika virus EDIII with CRM197, 8-arm PEG and mannan for development of an effective Zika virus vaccine[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,190:713-721. |
| APA | He, Yunxia,Yu, Weili,Xiao, Lucheng,Shen, Lijuan,Qi, Jinming,&Hu, Tao.(2021).Conjugation of Zika virus EDIII with CRM197, 8-arm PEG and mannan for development of an effective Zika virus vaccine.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,190,713-721. |
| MLA | He, Yunxia,et al."Conjugation of Zika virus EDIII with CRM197, 8-arm PEG and mannan for development of an effective Zika virus vaccine".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 190(2021):713-721. |
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