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Conjugation of Zika virus EDIII with CRM197, 8-arm PEG and mannan for development of an effective Zika virus vaccine
He, Yunxia1,2; Yu, Weili1; Xiao, Lucheng1,2; Shen, Lijuan1; Qi, Jinming1; Hu, Tao1
2021-11-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume190Pages:713-721
AbstractZika virus (ZIKV) induces neurological and autoimmune complications such as microcephaly and Guillain-Barre syndrome. Effective vaccines are necessary to prevent the ZIKV infection. E protein of ZIKV is responsible for virus attachment, entry, and fusion. The domain III of E protein (EDIII) contains the neutralizing epitopes and is ideal to act as an antigen for ZIKV vaccine. However, EDIII is poorly immunogenic. CRM197 is a carrier protein and can activate T helper cells for EDIII. Mannan is a ligand of TLR-4 or TLR-2. Eight-arm PEG can link multiple EDIII molecules in one entity. In the present study, EDIII was covalently conjugated with CRM197, 8-arm PEG and mannan to improve the immunogenicity of EDIII. The conjugate (CRM-EDIII-PM) elicited high EDIII-specific antibody titers in the BALB/c mice. Th1-type cytokines (IFN-gamma and IL-2) and Th2-type cytokines (IL-5 and IL10) were secreted at a marked level. Thus, CRM-EDIII-PM could stimulate potent humoral and cellular immune response to EDIII. The serum exposure of CRM-EDIII-PM to the immune system was prolonged. Moreover, CRM-EDIII-PM did not lead to apparent toxicity to the organs. Therefore, CRM-EDIII-PM was expected as a promising vaccine candidate for its ability to induce strong immune responses.
KeywordZika virus EDIII Adjuvant
DOI10.1016/j.ijbiomac.2021.08.177
Language英语
WOS KeywordIMMUNOGENICITY ; IMPROVES ; SAFETY
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000710145500008
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/50661
Collection中国科学院过程工程研究所
Corresponding AuthorQi, Jinming; Hu, Tao
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
He, Yunxia,Yu, Weili,Xiao, Lucheng,et al. Conjugation of Zika virus EDIII with CRM197, 8-arm PEG and mannan for development of an effective Zika virus vaccine[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,190:713-721.
APA He, Yunxia,Yu, Weili,Xiao, Lucheng,Shen, Lijuan,Qi, Jinming,&Hu, Tao.(2021).Conjugation of Zika virus EDIII with CRM197, 8-arm PEG and mannan for development of an effective Zika virus vaccine.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,190,713-721.
MLA He, Yunxia,et al."Conjugation of Zika virus EDIII with CRM197, 8-arm PEG and mannan for development of an effective Zika virus vaccine".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 190(2021):713-721.
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