CAS OpenIR
Bioemulsification and Microbial Community Reconstruction in Thermally Processed Crude Oil
Hu, Bing1,2; Zhao, Jie-Yu3; Nie, Yong3; Qin, Xiao-Yu3; Zhang, Kai-Duan3; Xing, Jian-Min4,5; Wu, Xiao-Lei3,6
2021-10-01
Source PublicationMICROORGANISMS
Volume9Issue:10Pages:18
AbstractUtilization of low-cost, environmental-friendly microbial enhanced oil recovery (MEOR) techniques in thermal recovery-processed oil reservoirs is potentially feasible. However, how exogenous microbes facilitate crude oil recovery in this deep biosphere, especially under mesophilic conditions, is scarcely investigated. In this study, a thermal treatment and a thermal recurrence were processed on crude oil collected from Daqing Oilfield, and then a 30-day incubation of the pretreated crude oil at 37 & DEG;C was operated with the addition of two locally isolated hydrocarbon-degrading bacteria, Amycolicicoccus subflavus DQS3-9A1(T) and Dietzia sp. DQ12-45-1b, respectively. The pH, surface tension, hydrocarbon profiles, culture-dependent cell densities and taxonomies, and whole and active microbial community compositions were determined. It was found that both A. subflavus DQS3-9A1(T) and Dietzia sp. DQ12-45-1b successfully induced culture acidification, crude oil bioemulsification, and residual oil sub-fraction alteration, no matter whether the crude oil was thermally pretreated or not. Endogenous bacteria which could proliferate on double heated crude oil were very few. Compared with A. subflavus, Dietzia sp. was substantially more effective at inducing the proliferation of varied species in one-time heated crude oil. Meanwhile, the effects of Dietzia sp. on crude oil bioemulsification and hydrocarbon profile alteration were not significantly influenced by the ploidy increasing of NaCl contents (from 5 g/L to 50 g/L), but the reconstructed bacterial communities became very simple, in which the Dietzia genus was predominant. Our study provides useful information to understand MEOR trials on thermally processed oil reservoirs, and proves that this strategy could be operated by using the locally available hydrocarbon-degrading microbes in mesophilic conditions with different salinity degrees.

Keywordmicrobial enhanced oil recovery (MEOR) heat perturbation bioemulsification microbial community reconstruction hypersalinity
DOI10.3390/microorganisms9102054
Language英语
WOS KeywordR-SELECTION ; RECOVERY ; DEGRADATION ; HYDROCARBONS ; SUBSURFACE
Funding ProjectNational Key R&D Program of China[2018YFA0902100] ; National Key R&D Program of China[2020YFA0908300] ; National Natural Science Foundation of China[22178024] ; Open Funding Project of the State Key Laboratory of Biochemical Engineering[2020KF-05]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
Funding OrganizationNational Key R&D Program of China ; National Natural Science Foundation of China ; Open Funding Project of the State Key Laboratory of Biochemical Engineering
WOS IDWOS:000711398300001
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/50686
Collection中国科学院过程工程研究所
Corresponding AuthorNie, Yong; Wu, Xiao-Lei
Affiliation1.Beijing Inst Technol, Coll Chem & Chem Engn, Dept Chem Engn, Grp Biochem Engn, Beijing 102401, Peoples R China
2.Minist Ind & Informat Technol China, Key Lab Med Mol Sci & Pharmaceut Engn, Beijing 102401, Peoples R China
3.Peking Univ, Coll Engn, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, CAS Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
6.Peking Univ, Inst Ecol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Hu, Bing,Zhao, Jie-Yu,Nie, Yong,et al. Bioemulsification and Microbial Community Reconstruction in Thermally Processed Crude Oil[J]. MICROORGANISMS,2021,9(10):18.
APA Hu, Bing.,Zhao, Jie-Yu.,Nie, Yong.,Qin, Xiao-Yu.,Zhang, Kai-Duan.,...&Wu, Xiao-Lei.(2021).Bioemulsification and Microbial Community Reconstruction in Thermally Processed Crude Oil.MICROORGANISMS,9(10),18.
MLA Hu, Bing,et al."Bioemulsification and Microbial Community Reconstruction in Thermally Processed Crude Oil".MICROORGANISMS 9.10(2021):18.
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