CAS OpenIR  > 生化工程国家重点实验室
Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase
Wang, Jun1,2; Hu, Tao1; Liu, Yongdong1; Zhang, Guifeng1; Ma, Guanghui1; Su, Zhiguo1; Hu, T
2011-05-01
Source PublicationANALYTICAL BIOCHEMISTRY
ISSN0003-2697
Volume412Issue:1Pages:114-116
AbstractStaphylokinase (SAK) is a therapeutic protein with promise for thrombolytic therapy of acute myocardial infarction. In this study, polyethylene glycol (PEG) aldehyde was used for N-terminal PEGylation of SAK to improve the pharmacological profiles of SAK. Due to the presence of the competitive PEGylation between the N terminus and the Lys residues, kinetic and stoichiometric analysis was carried out to investigate the process for the N-terminal PEGylation of SAK. To achieve this objective, size exclusion chromatography and tryptic peptide mapping were used to measure the PEGylation extent of SAIL molecule and its specific amino acid residues, respectively. (C) 2010 Elsevier Inc. All rights reserved.
KeywordRecombinant Staphylokinase Poly(Ethylene Glycol) Proteins Plasminogen
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.ab.2010.12.030
Indexed BySCI
Language英语
WOS KeywordRECOMBINANT STAPHYLOKINASE ; POLY(ETHYLENE GLYCOL) ; PROTEINS ; PLASMINOGEN
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
WOS IDWOS:000288589700018
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5068
Collection生化工程国家重点实验室
Corresponding AuthorHu, T
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jun,Hu, Tao,Liu, Yongdong,et al. Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase[J]. ANALYTICAL BIOCHEMISTRY,2011,412(1):114-116.
APA Wang, Jun.,Hu, Tao.,Liu, Yongdong.,Zhang, Guifeng.,Ma, Guanghui.,...&Hu, T.(2011).Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase.ANALYTICAL BIOCHEMISTRY,412(1),114-116.
MLA Wang, Jun,et al."Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase".ANALYTICAL BIOCHEMISTRY 412.1(2011):114-116.
Files in This Item:
File Name/Size DocType Version Access License
20131106-979-复件 1-s2(475KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Jun]'s Articles
[Hu, Tao]'s Articles
[Liu, Yongdong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Jun]'s Articles
[Hu, Tao]'s Articles
[Liu, Yongdong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Jun]'s Articles
[Hu, Tao]'s Articles
[Liu, Yongdong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.